修饰的多核苷酸。IV. 含5-烷基尿嘧啶的聚[d(A-r5U)]共聚物对DNA和RNA聚合酶的模板活性。
Modified polynucleotides. IV. Template activity of 5-alkyluracil-containing poly [d(A-r5U)] copolymers for DNA and RNA polymerases.
作者信息
Sági J, Otvös L
出版信息
Nucleic Acids Res. 1979 Nov 24;7(6):1593-601. doi: 10.1093/nar/7.6.1593.
Abstract
The change of templating properties for E. coli DNA and RNA polymerases of poly[d(A-r5U)] copolymers on substitution of the uracil for 5-methyl-, 5-ethyl-, 5-n-propyl-, 5-n-butyl- or 5-n-pentyluracil, resp., was studied. All modified polymers proved to be more effective template-primers than poly[d(A-U)] or poly[d(A-T)] for the DNA polymerase. Direct dependence of the template-primer efficiency (vmax/Km) value on the thermal stability of the polymers was observed. For transcription the r = ethyl and n-propyl polymers were also more effective templates than poly[d(A-T)].
摘要
研究了用5-甲基尿嘧啶、5-乙基尿嘧啶、5-正丙基尿嘧啶、5-正丁基尿嘧啶或5-正戊基尿嘧啶分别取代尿嘧啶后，聚[d(A-r5U)]共聚物对大肠杆菌DNA和RNA聚合酶模板性质的影响。所有修饰后的聚合物对DNA聚合酶而言，都比聚[d(A-U)]或聚[d(A-T)]更有效地作为模板引物。观察到模板引物效率（vmax/Km）值与聚合物热稳定性之间存在直接相关性。对于转录而言，r = 乙基和正丙基的聚合物也是比聚[d(A-T)]更有效的模板。
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Biochemistry. 1973 Jan 2;12(1):151-7. doi: 10.1021/bi00725a025.
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