Department of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen Friedrich-Alexander-University of Erlangen-Nürnberg (FAU), Krankenhausstraße 12, 91054, Erlangen, Germany.
Institute of Polymer Materials, Friedrich-Alexander-University of Erlangen-Nürnberg (FAU), Martensstrasse 7, 91058, Erlangen, Germany.
J Mater Sci Mater Med. 2020 Feb 3;31(2):23. doi: 10.1007/s10856-020-6360-5.
Capsular contracture remains a challenge in plastic surgery and represents one of the most common postoperative complications following alloplastic breast reconstruction. The impact of the surface structure of silicone implants on the foreign body reaction and the behaviour of connective tissue-producing cells has already been discussed. The aim of this study was to investigate different pore sizes of silicone surfaces and their influence on human fibroblasts in an in vitro model. Four different textures (no, fine, medium and coarse texture) produced with the salt-loss technique, have been assessed in an in vitro model. Human fibroblasts were seeded onto silicone sheets and evaluated after 1, 4 and 7 days microscopically, with viability assay and gene expression analysis. Comparing the growth behaviour and adhesion of the fibroblasts on the four different textures, a dense cell layer, good adhesion and bridge-building ability of the cells could be observed for the fine and medium texture. Cell number and viability of the cells were increasing during the time course of experiments on every texture. TGFß1 was lowest expressed on the fine and medium texture indicating a trend for decreased fibrotic activity. For silicone surfaces produced with the salt-loss technique, we were able to show an antifibrotic effect of smaller sized pores. These findings underline the hypothesis of a key role of the implant surface and the pore size and pore structure in preventing capsular contracture.
包膜挛缩仍然是整形外科学的一个挑战，是所有假体乳房重建术后最常见的并发症之一。硅酮植入物的表面结构对异物反应和产生结缔组织的细胞行为的影响已经被讨论过了。本研究的目的是在体外模型中研究不同的硅酮表面孔径及其对人成纤维细胞的影响。通过盐损耗技术制作的四种不同纹理（无纹理、细纹理、中纹理和粗纹理）在体外模型中进行了评估。将人成纤维细胞接种到硅酮片上，在 1、4 和 7 天进行显微镜观察、活力测定和基因表达分析。比较四种不同纹理上成纤维细胞的生长行为和黏附性，可观察到细纹理和中纹理具有致密的细胞层、良好的黏附性和细胞桥接能力。在每个纹理的实验过程中，细胞数量和活力都在增加。TGFß1 在细纹理和中纹理上的表达最低，表明纤维化活性降低的趋势。对于用盐损耗技术制作的硅酮表面，我们能够证明较小孔径具有抗纤维化作用。这些发现支持了这样一种假设，即植入物表面以及孔径和孔结构在防止包膜挛缩方面起着关键作用。
