Anthony A, Dolber P C, Romanosky A J
J Histochem Cytochem. 1977 Apr;25(4):287-94. doi: 10.1177/25.4.67138.
Feulgen deoxyribonucleic acid cytophotometry of Papanicolaou destained specimens revealed a differential loss in Feulgen reactivity among human buccal and cervical smears, cultured embryonic lung fibroblasts and invasive cervical carcinoma cells. Loss in Feulgen reactivity in Papanicolaou destained fibroblasts and polyploid nuclei of malignant lesions was observed to result in underestimates of relative Feulgen deoxyribonucleic acid and nuclear area values using scanning integrating microdensitometry. Thus, Papanicolaou stained preparations may not be suitable for deoxyribonucleic acid quantification of high ploidy lesions since distributional absorption error is unpredictably influenced by such factors as ploidy level, nuclear size, chromatin dispersion and differential aldehyde loss during destaining. Feulgen deoxyribonucleic acid cytophotometry of Papanicolaou stained preparations can be useful for differentiating benign from malignant lesions if extent of aneuploidy (as reflected in abnormal deoxyribonucleic acid frequency distribution profile) is used as a diagnostic indicator.
对帕帕尼科拉乌氏染色标本进行福尔根脱氧核糖核酸细胞光度测定发现，在人类颊部和宫颈涂片、培养的胚胎肺成纤维细胞以及浸润性宫颈癌细胞中，福尔根反应性存在差异丧失。观察到在帕帕尼科拉乌氏染色的成纤维细胞和恶性病变的多倍体细胞核中，福尔根反应性丧失导致使用扫描积分显微密度计低估了相对福尔根脱氧核糖核酸和核面积值。因此，帕帕尼科拉乌氏染色制剂可能不适用于高倍体病变的脱氧核糖核酸定量，因为分布吸收误差会受到诸如倍体水平、核大小、染色质分散以及脱色过程中醛的差异丧失等因素的不可预测影响。如果将非整倍体程度（如异常脱氧核糖核酸频率分布曲线所反映）用作诊断指标，对帕帕尼科拉乌氏染色制剂进行福尔根脱氧核糖核酸细胞光度测定可有助于区分良性和恶性病变。
