Shaikh Saame Raza, Sullivan E Madison, Alleman Rick J, Brown David A, Zeczycki Tonya N
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University , Greenville, North Carolina 27834, United States.
Biochemistry. 2014 Sep 9;53(35):5589-91. doi: 10.1021/bi500868g. Epub 2014 Aug 26.
Activities of the enzymes involved in cellular respiration are markedly influenced by the composition of the phospholipid environment of the inner mitochondrial membrane. Contrary to previous suppositions, we show that fusion of mitochondria isolated from healthy cardiac muscle with cardiolipin or dioleoylphosphatidylcholine results in a 2-6-fold reduction in the activity of complexes I, II, and IV. The activity of complex III was unaffected by increased phospholipid levels. Phospholipid content had an indiscriminate yet detrimental effect on the combined activities of complexes I+III and II+III. These results have strong implications for therapeutic lipid replacement strategies, in which phospholipid modification of the mitochondria is proposed to enhance mitochondrial function.
参与细胞呼吸的酶的活性受到线粒体内膜磷脂环境组成的显著影响。与先前的推测相反，我们发现从健康心肌中分离出的线粒体与心磷脂或二油酰磷脂酰胆碱融合后，复合物I、II和IV的活性降低了2至6倍。复合物III的活性不受磷脂水平升高的影响。磷脂含量对复合物I + III和II + III的联合活性有不加区分但有害的影响。这些结果对治疗性脂质替代策略具有重要意义，在这些策略中，有人提出对线粒体进行磷脂修饰以增强线粒体功能。
