Tian Tian, Cai Xiaojing, Zhu Huang
J Tradit Chin Med. 2016 Oct;36(5):678-82. doi: 10.1016/s0254-6272(16)30089-9.
To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen (Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields (ELF-EMFs).
Human fetal scleral fibroblasts (HFSFs) were divided into 5 groups: (a) untreated controls; (b) cells treated with ELF-EMFs; (c) cells treated with ELF-EMFs and puerarin 0.1 μM; (d) cells treated with ELF-EMFs and puerarin 1 μM; (e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2 (MMP-2) activity was measured by gelatin enzymography. MMP-2 and collagenⅠ(COL1A1) mRNA, protein expression were measured by Real-Time polymerase chain reaction , Western blot analysis, respectively.
Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, mRNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 mRNA and protein expression.
Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.
评估葛根素[从葛根（野葛）中提取的一种异黄酮化合物]在极低频电磁场（ELF-EMFs）诱导的巩膜重塑中的保护作用。
将人胎儿巩膜成纤维细胞（HFSFs）分为5组：（a）未处理的对照组；（b）用ELF-EMFs处理的细胞；（c）用ELF-EMFs和0.1μM葛根素处理的细胞；（d）用ELF-EMFs和1μM葛根素处理的细胞；（e）用ELF-EMFs和10μM葛根素处理的细胞。通过细胞计数试剂盒-8法测定细胞增殖活性。通过明胶酶谱法测定基质金属蛋白酶-2（MMP-2）活性。分别通过实时聚合酶链反应、蛋白质免疫印迹分析测定MMP-2和Ⅰ型胶原（COL1A1）的mRNA、蛋白表达。
葛根素减轻了ELF-EMFs对HFSFs细胞增殖、MMP-2活性、mRNA及蛋白表达的抑制作用，并增强了COL1A1的mRNA和蛋白表达。
发现葛根素参与了基质重塑过程。它可能是治疗与眼部疾病相关的巩膜细胞外基质降解的一种潜在药物。
