J Tradit Chin Med. 2013 Oct;33(5):664-8. doi: 10.1016/s0254-6272(14)60039-x.
To study the effect of puerarin on matrix metalloproteinase-2 (MMP-2) gene and protein expression in human fetal scleral fibroblasts (HFSFs) exposed to extremely low frequency electromagnetic fields (ELF-EMF).
Cultured HFSFs were exposed to 0.2 mT ELF-EMF for 24 h. The experimental groups were divided into subgroups treated with 0, 0.1, 1 and 10 microM puerarin respectively. The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.
MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for 24 h. Puerarin at the concentration of 0.1 microM reversed this effect by 8.53% in mRNA and by 17.97% in protein expression (P < 0.05). The effect was more prominent at higher concentrations (1 and 10 microM, P < 0.01).
Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells. Puerarin reversed the action to some extent in a specific concentration range. Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
研究葛根素对人胎儿巩膜成纤维细胞（HFSFs）暴露于极低频电磁场（ELF-EMF）后基质金属蛋白酶-2（MMP-2）基因和蛋白表达的影响。
培养的 HFSFs 暴露于 0.2 mT 的 ELF-EMF 中 24 小时。实验组分为分别用 0、0.1、1 和 10 μM 葛根素处理的亚组。分别用实时聚合酶链反应和 Western blot 分析检测 MMP-2 mRNA 和蛋白的表达。
在 0.2 mT 磁通密度下暴露 24 小时，MMP-2 mRNA 和蛋白表达分别增加了 0.793 倍和 1.130 倍。浓度为 0.1 μM 的葛根素使 mRNA 表达降低了 8.53%，蛋白表达降低了 17.97%（P < 0.05）。在更高浓度（1 和 10 μM）时，效果更为显著（P < 0.01）。
暴露于 ELF-EMF 会增加 HFSF 细胞中 MMP-2 mRNA 和蛋白的表达。葛根素在特定浓度范围内以一定程度逆转了这种作用。我们的结果表明，葛根素可能保护巩膜组织免受 ELF-EMF 暴露引起的表达增加。
