Borhani Katayoun, Ahmadi Ali, Rahimi Fateh, Pourshafie Mohammad Reza, Talebi Malihe
Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran.
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
Jundishapur J Microbiol. 2014 Feb;7(2):e8951. doi: 10.5812/jjm.8951. Epub 2014 Feb 1.
Sewage treatment plants are considered to be the hotspots for antibiotic resistance transfer among bacterial species. Many fecal bacteria including Enterococci circulate and are exposed to antibiotic residues in this environment. Being as one of the most common cause of nosocomial infections, special concerns have risen worldwide about the rate and characteristics of Enterococci (especially, isolates with high resistance against glycopeptides) which are available in raw sewages.
Study on the vancomycin Resistant E. faecium diversity in Tehran sewage by plasmid profile, biochemical fingerprinting and antibiotic resistance.
Forty isolates recovered from an urban sewage treatment plant were studied during 2009- 2010. The antibiotic resistance of isolates against 7 antibiotics was examined by disk diffusion method. Extraction of plasmid DNA was performed and identification of van genotype (vanA and vanB) was done by PCR. Biochemical fingerprinting was done by the use of Phene-Plate system (PhP).
All isolates were found to be resistant to erythromycin, ampicillin and ciprofloxacin. The PCR analyses showed that all E. faecium isolates harbored vanA gene and 5 (13%) isolates harbored vanA and vanB concomitantly. By plasmid profiling the VRE isolates differentiated into 11 types. PhP showed that VRE isolates were grouped into 23 biochemical types.
The combination of plasmid profiling and PhP techniques revealed the presence of diverse population of VRE in sewage treatment plant in Tehran. Furthermore, the results showed that the PhP technique is a reliable method in determining the VRE clonal diversity.
污水处理厂被认为是细菌物种间抗生素耐药性转移的热点区域。许多包括肠球菌在内的粪便细菌在这种环境中循环并接触抗生素残留。作为医院感染最常见的原因之一，全球对原污水中存在的肠球菌（尤其是对糖肽类具有高耐药性的分离株）的发生率和特征给予了特别关注。
通过质粒图谱、生化指纹分析和抗生素耐药性研究德黑兰污水中耐万古霉素屎肠球菌的多样性。
2009 - 2010年期间对从城市污水处理厂分离出的40株菌株进行了研究。采用纸片扩散法检测分离株对7种抗生素的耐药性。进行质粒DNA提取，并通过聚合酶链反应（PCR）鉴定van基因型（vanA和vanB）。使用Phene - Plate系统（PhP）进行生化指纹分析。
所有分离株均对红霉素、氨苄西林和环丙沙星耐药。PCR分析表明，所有屎肠球菌分离株均携带vanA基因，5株（13%）分离株同时携带vanA和vanB基因。通过质粒图谱分析，耐万古霉素肠球菌分离株分为11种类型。PhP显示耐万古霉素肠球菌分离株分为23种生化类型。
质粒图谱分析和PhP技术相结合揭示了德黑兰污水处理厂中存在多种耐万古霉素肠球菌群体。此外，结果表明PhP技术是确定耐万古霉素肠球菌克隆多样性的可靠方法。
