Sun Zheng, Li Mingji, Qi Qingsheng, Gao Cuijuan, Lin Carol Sze Ki
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, People's Republic of China.
Appl Biochem Biotechnol. 2014 Nov;174(5):1822-33. doi: 10.1007/s12010-014-1169-7. Epub 2014 Aug 23.
Mixed food waste, which was directly collected from restaurants without pretreatments, was used as a valuable feedstock in succinic acid (SA) fermentation in the present study. Commercial enzymes and crude enzymes produced from Aspergillus awamori and Aspergillus oryzae were separately used in hydrolysis of food waste, and their resultant hydrolysates were evaluated. For hydrolysis using the fungal mixture comprising A. awamori and A. oryzae, a nutrient-complete food waste hydrolysate was generated, which contained 31.9 g L(-1) glucose and 280 mg L(-1) free amino nitrogen. Approximately 80-90 % of the solid food waste was also diminished. In a 2.5 L fermentor, 29.9 g L(-1) SA was produced with an overall yield of 0.224 g g(-1) substrate using food waste hydrolysate and recombinant Escherichia coli. This is comparable to many similar studies using various wastes or by-products as substrates. Results of this study demonstrated the enormous potential of food waste as renewable resource in the production of bio-based chemicals and materials via microbial bioconversion.
本研究中，未经预处理直接从餐馆收集的混合食物垃圾被用作琥珀酸（SA）发酵中有价值的原料。分别使用商业酶以及由泡盛曲霉和米曲霉产生的粗酶对食物垃圾进行水解，并对其水解产物进行评估。对于使用包含泡盛曲霉和米曲霉的真菌混合物进行的水解，生成了营养成分完整的食物垃圾水解产物，其中含有31.9 g L⁻¹葡萄糖和280 mg L⁻¹游离氨基氮。大约80 - 90%的固体食物垃圾也被减少。在一个2.5 L的发酵罐中，使用食物垃圾水解产物和重组大肠杆菌生产出了29.9 g L⁻¹的SA，底物总产率为0.224 g g⁻¹。这与许多使用各种废物或副产品作为底物的类似研究相当。本研究结果证明了食物垃圾作为可再生资源通过微生物生物转化生产生物基化学品和材料的巨大潜力。
