Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Material Science, Michigan State University (MSU), 3900 Collins Road, MBI Building, Lansing, MI 48910, United States.
Government College of Technology, Coimbatore 641013, Tamil Nadu, India.
Waste Manag. 2017 Apr;62:247-254. doi: 10.1016/j.wasman.2017.02.013. Epub 2017 Feb 20.
Sugar-rich food waste is a sustainable feedstock that can be converted into ethanol without an expensive thermochemical pretreatment that is commonly used in first and second generation processes. In this manuscript we have outlined the pie waste conversion to ethanol through a two-step process, namely, enzyme hydrolysis using commercial enzyme products mixtures and microbial fermentation using yeast. Optimized enzyme cocktail was found to be 45% alpha amylase, 45% gamma amylase, and 10% pectinase at 2.5mg enzyme protein/g glucan produced a hydrolysate with high glucose concentration. All three solid loadings (20%, 30%, and 40%) produced sugar-rich hydrolysates and ethanol with little to no enzyme or yeast inhibition. Enzymatic hydrolysis and fermentation process mass balance was carried out using pie waste on a 1000g dry weight basis that produced 329g ethanol at 20% solids loading. This process clearly demonstrate how food waste could be efficiently converted to ethanol that could be used for making biodiesel by reacting with waste cooking oil.
富含糖分的食物废料是一种可持续的原料，可以转化为乙醇，而无需昂贵的热化学预处理，这种预处理通常用于第一代和第二代工艺。在本文中，我们概述了通过两步法将馅饼废料转化为乙醇，即使用商业酶产品混合物进行酶水解，以及使用酵母进行微生物发酵。优化的酶混合物为 45%的α-淀粉酶、45%的γ-淀粉酶和 10%的果胶酶，每克葡聚糖产生 2.5mg 酶蛋白，可得到高葡萄糖浓度的水解产物。所有三种固体负荷（20%、30%和 40%）都产生了富含糖分的水解产物和乙醇，几乎没有酶或酵母抑制。在 1000g 干重的基础上，使用馅饼废料进行了酶解和发酵过程的质量平衡，在 20%的固体负荷下可生产 329g 乙醇。该工艺清楚地表明，食物废料可以通过与废食用油反应转化为乙醇，然后用于制造生物柴油，从而实现高效转化。
