Gallo P, Tavolato B, Bergenbrant S, Sidén A
Department of Neurology, University of Padova, Italy.
J Neurol Sci. 1989 Dec;94(1-3):241-53. doi: 10.1016/0022-510x(89)90234-7.
The immunoglobulin light chain patterns were studied in paired cerebrospinal fluid (CSF) and serum samples from 30 controls, 70 multiple sclerosis (MS) patients, 18 subjects with other inflammatory neurological diseases and 17 patients with other non-inflammatory neurological disorders. In MS, all CSF samples examined by two-dimensional gel electrophoresis exhibited clonally restricted light chain components. Isoelectric focusing and immunoblotting detected free light chains in around 90% of CSF samples from patients with MS or other inflammatory diseases. These components were clonally restricted, appeared in both mono- and dimeric forms and occurred in CSF samples with as well as without oligoclonal immunoglobulin G bands. Generally, the positive CSF samples contained kappa as well as lambda free lights chains. Such components were not detected in the sera, nor in the CSF samples from controls or patients with non-inflammatory diseases.
对30名对照者、70名多发性硬化症（MS）患者、18名患有其他炎性神经系统疾病的受试者以及17名患有其他非炎性神经系统疾病的患者的配对脑脊液（CSF）和血清样本中的免疫球蛋白轻链模式进行了研究。在MS患者中，通过二维凝胶电泳检测的所有脑脊液样本均显示出克隆受限的轻链成分。等电聚焦和免疫印迹法在约90%的MS患者或其他炎性疾病患者的脑脊液样本中检测到游离轻链。这些成分是克隆受限的，以单体和二聚体形式出现，并且在有和没有寡克隆免疫球蛋白G带的脑脊液样本中均有出现。一般来说，阳性脑脊液样本中同时含有κ和λ游离轻链。在血清中，以及对照者或非炎性疾病患者的脑脊液样本中均未检测到此类成分。
