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超分辨率分子与功能成像在生命科学和材料科学中的纳米级结构研究

Super-resolution molecular and functional imaging of nanoscale architectures in life and materials science.

机构信息

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology , Jeddah , Saudi Arabia.

出版信息

Front Bioeng Biotechnol. 2014 Jun 12;2:20. doi: 10.3389/fbioe.2014.00020. eCollection 2014.

Abstract

Super-resolution (SR) fluorescence microscopy has been revolutionizing the way in which we investigate the structures, dynamics, and functions of a wide range of nanoscale systems. In this review, I describe the current state of various SR fluorescence microscopy techniques along with the latest developments of fluorophores and labeling for the SR microscopy. I discuss the applications of SR microscopy in the fields of life science and materials science with a special emphasis on quantitative molecular imaging and nanoscale functional imaging. These studies open new opportunities for unraveling the physical, chemical, and optical properties of a wide range of nanoscale architectures together with their nanostructures and will enable the development of new (bio-)nanotechnology.

摘要

超分辨率(SR)荧光显微镜技术正在彻底改变我们研究各种纳米尺度系统的结构、动态和功能的方式。在这篇综述中,我描述了各种 SR 荧光显微镜技术的现状,以及用于 SR 显微镜的荧光染料和标记物的最新进展。我讨论了 SR 显微镜在生命科学和材料科学领域的应用,特别强调定量分子成像和纳米尺度功能成像。这些研究为揭示广泛的纳米尺度结构及其纳米结构的物理、化学和光学性质开辟了新的机会,并将推动新的(生物)纳米技术的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8784/4126472/59e7a8e2a3c3/fbioe-02-00020-g001.jpg

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