Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology , Jeddah , Saudi Arabia.
Front Bioeng Biotechnol. 2014 Jun 12;2:20. doi: 10.3389/fbioe.2014.00020. eCollection 2014.
Super-resolution (SR) fluorescence microscopy has been revolutionizing the way in which we investigate the structures, dynamics, and functions of a wide range of nanoscale systems. In this review, I describe the current state of various SR fluorescence microscopy techniques along with the latest developments of fluorophores and labeling for the SR microscopy. I discuss the applications of SR microscopy in the fields of life science and materials science with a special emphasis on quantitative molecular imaging and nanoscale functional imaging. These studies open new opportunities for unraveling the physical, chemical, and optical properties of a wide range of nanoscale architectures together with their nanostructures and will enable the development of new (bio-)nanotechnology.
超分辨率(SR)荧光显微镜技术正在彻底改变我们研究各种纳米尺度系统的结构、动态和功能的方式。在这篇综述中,我描述了各种 SR 荧光显微镜技术的现状,以及用于 SR 显微镜的荧光染料和标记物的最新进展。我讨论了 SR 显微镜在生命科学和材料科学领域的应用,特别强调定量分子成像和纳米尺度功能成像。这些研究为揭示广泛的纳米尺度结构及其纳米结构的物理、化学和光学性质开辟了新的机会,并将推动新的(生物)纳米技术的发展。