Nandi Somen, Caicedo Karen, Cognet Laurent
Laboratoire Photonique Numérique et Nanosciences, Université de Bordeaux, UMR 5298, 33400 Talence, France.
Institut d'Optique and CNRS, LP2N UMR 5298, 33400 Talence, France.
Nanomaterials (Basel). 2022 Apr 22;12(9):1433. doi: 10.3390/nano12091433.
We recently assisted in a revolution in the realm of fluorescence microscopy triggered by the advent of super-resolution techniques that surpass the classic diffraction limit barrier. By providing optical images with nanometer resolution in the far field, super-resolution microscopy (SRM) is currently accelerating our understanding of the molecular organization of bio-specimens, bridging the gap between cellular observations and molecular structural knowledge, which was previously only accessible using electron microscopy. SRM mainly finds its roots in progress made in the control and manipulation of the optical properties of (single) fluorescent molecules. The flourishing development of novel fluorescent nanostructures has recently opened the possibility of associating super-resolution imaging strategies with nanomaterials' design and applications. In this review article, we discuss some of the recent developments in the field of super-resolution imaging explicitly based on the use of nanomaterials. As an archetypal class of fluorescent nanomaterial, we mainly focus on single-walled carbon nanotubes (SWCNTs), which are photoluminescent emitters at near-infrared (NIR) wavelengths bearing great interest for biological imaging and for information optical transmission. Whether for fundamental applications in nanomaterial science or in biology, we show how super-resolution techniques can be applied to create nanoscale images "", "" and "" SWCNTs.
我们最近助力了一场荧光显微镜领域的革命,这场革命由超分辨率技术的出现所引发,该技术突破了经典的衍射极限障碍。通过在远场提供纳米分辨率的光学图像,超分辨率显微镜(SRM)目前正在加速我们对生物样本分子组织的理解,弥合细胞观测与分子结构知识之间的差距,而此前这只能通过电子显微镜才能实现。SRM主要源于在(单个)荧光分子光学性质的控制和操纵方面所取得的进展。新型荧光纳米结构的蓬勃发展最近开启了将超分辨率成像策略与纳米材料的设计及应用相结合的可能性。在这篇综述文章中,我们明确基于纳米材料的使用来讨论超分辨率成像领域的一些最新进展。作为一类典型的荧光纳米材料,我们主要聚焦于单壁碳纳米管(SWCNT),它是近红外(NIR)波长的光致发光发射体,在生物成像和信息光传输方面极具吸引力。无论是在纳米材料科学还是生物学的基础应用中,我们都展示了超分辨率技术如何能够用于创建纳米尺度的图像“”“”和单壁碳纳米管。