Muséum National d'Histoire Naturelle, UMR MNHN-CNRS 7245, 12 rue Buffon, 75231 Paris Cedex 05, France.
Université de Tours-INRA, UMR 1282, Faculté de Pharmacie, 31 Avenue Monge, 37200 Tours Cedex, France.
Toxins (Basel). 2014 Aug 21;6(8):2541-67. doi: 10.3390/toxins6082541.
Serum therapy remains the only specific treatment against envenoming, but anti-venoms are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum. Most of these anti-venoms are considered to be efficient, but their production is tedious, and their use may be associated with adverse effects. Recombinant antibodies and smaller functional units are now emerging as credible alternatives and constitute a source of still unexploited biomolecules capable of neutralizing venoms. This review will be a walk through the technologies that have recently been applied leading to novel antibody formats with better properties in terms of homogeneity, specific activity and possible safety.
血清疗法仍然是唯一针对中毒的特异性治疗方法，但抗毒液仍然是通过从高度免疫的马血清中分离的多克隆抗体进行片段化制备的。大多数这些抗毒液被认为是有效的，但它们的生产繁琐，并且其使用可能与不良反应有关。重组抗体和更小的功能单位现在作为可信的替代品出现，并且是能够中和毒液的仍然未被开发的生物分子的来源。这篇综述将带您了解最近应用的技术，这些技术导致了新型抗体形式的出现，这些新型抗体在均一性、特异性活性和可能的安全性方面具有更好的特性。
