Hovdenes J
Institute of Immunology and Rheumatology, National Hospital of Norway, Oslo.
Scand J Rheumatol. 1989;18(6):385-92. doi: 10.3109/03009748909102100.
The purpose of this study was to compare CD4+ cells from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis with regard to mitogen-induced production of B-cell growth-promoting activity. CD4+ cells were isolated by a direct immunomagnetic technique and supernatants from both unstimulated and mitogen-stimulated CD4+ cells were studied. B-cell growth-promoting activity was assayed using highly purified B cells obtained from peripheral blood of healthy individuals. The indicator B cells were isolated by an indirect immunomagnetic technique and solid-phase anti-mu was used for activation of the B cells. Supernatants of unstimulated CD4+ cells from SF and PB did not contain B-cell growth-promoting activity, while usually high levels of B-cell growth-promoting activity were detected in the supernatants from mitogen-stimulated cultures. There was no significant difference in the B-cell growth-promoting activity level between supernatants from SF CD4+ and patient PB CD4+ cells, nor was there any significant difference between SF CD4+ and control PB CD4+ supernatants. The results indicate that the CD4+ cells in the SF have a normal potential for producing B-cell growth-promoting activity.
本研究的目的是比较类风湿性关节炎患者外周血（PB）和滑液（SF）中的CD4 +细胞在有丝分裂原诱导下产生B细胞生长促进活性的情况。通过直接免疫磁珠技术分离CD4 +细胞，并对未刺激和有丝分裂原刺激的CD4 +细胞的上清液进行研究。使用从健康个体外周血中获得的高度纯化的B细胞测定B细胞生长促进活性。通过间接免疫磁珠技术分离指示性B细胞，并使用固相抗μ激活B细胞。来自SF和PB的未刺激CD4 +细胞的上清液不含有B细胞生长促进活性，而在有丝分裂原刺激培养物的上清液中通常检测到高水平的B细胞生长促进活性。来自SF CD4 +和患者PB CD4 +细胞的上清液之间的B细胞生长促进活性水平没有显著差异，SF CD4 +和对照PB CD4 +上清液之间也没有任何显著差异。结果表明，SF中的CD4 +细胞具有产生B细胞生长促进活性的正常潜力。
