Kurantsin-Mills J, Jacobs H M, Siegel R, Cassidy M M, Lessin L S
Department of Physiology, George Washington University Medical Center, Washington, DC 20037.
Int J Rad Appl Instrum B. 1989;16(8):821-7. doi: 10.1016/0883-2897(89)90167-0.
Indium-111 oxine label erythrocytes are useful in scintigraphic studies of splenic function because of the high yield of gamma-photons [172(90%) and 247(94%) keV] of indium-111. However, the effects of indium-111 oxine on the structural and functional integrity of erythrocytes which might influence their reticulo-endothelial (RE) sequestration are unknown. We examined the morphology of human and rat indium-111 labeled erythrocytes by SEM, the distribution of the label within the cell by analysis of the membrane and cytosol (hemoglobin solution) and the kinetics of efflux of indium-111 from erythrocytes incubated at 37 degrees C in plasma or physiological buffer. Indium-111 oxine labeled red cells retain their discocytic morphology and the cell indices, and density characteristics on phthalate ester are similar to those of the control cells. The efficiency of labeling may be as high as 97%. Human or rat erythrocyte membranes retain 33 and 41% of indium-111, and the cytosol contains 67 and 59%, respectively. About 98% of the indium-111 is bound to the membrane proteins and 1% to the lipid bilayer. Efflux of indium-111 from cells in autologous plasma showed a multiphasic release resulting in about 4-5% release of the label in 2 h and 11.5% in 20 h. Cells in PBS showed 1-5% release of the label during the incubation period. These findings suggest that indium-111 oxine labeling of erythrocytes does not grossly alter the structural and deformability integrity of the cells to induce selective RE sequestration, unless the cells have been damaged prior to or during the labeling procedure, or the spleen is hyperactive.
由于铟 - 111具有高产率的γ光子（172keV，产率90%；247keV，产率94%），铟 - 111奥克辛标记的红细胞在脾功能闪烁显像研究中很有用。然而，铟 - 111奥克辛对红细胞结构和功能完整性的影响尚不清楚，而这种影响可能会影响其网状内皮（RE）滞留。我们通过扫描电子显微镜（SEM）检查了人和大鼠铟 - 111标记红细胞的形态，通过分析细胞膜和细胞溶质（血红蛋白溶液）来确定标记物在细胞内的分布，并研究了在37℃下于血浆或生理缓冲液中孵育的红细胞中铟 - 111的外排动力学。铟 - 111奥克辛标记的红细胞保持其双凹圆盘状形态以及细胞指数，并且在邻苯二甲酸酯上的密度特征与对照细胞相似。标记效率可能高达97%。人或大鼠红细胞膜保留33%和41%的铟 - 111，而细胞溶质分别含有67%和59%。约98%的铟 - 111与膜蛋白结合，1%与脂质双层结合。铟 - 111从自体血浆中的细胞外排呈现多相释放，在2小时内约有4 - 5%的标记物释放，20小时内释放11.5%。在磷酸盐缓冲液（PBS）中的细胞在孵育期间显示1 - 5%的标记物释放。这些发现表明，铟 - 111奥克辛对红细胞的标记不会严重改变细胞的结构和变形性完整性以诱导选择性RE滞留，除非细胞在标记过程之前或期间已受损，或者脾脏功能亢进。
