Archibald A R, Glassey K, Green R S, Lang W K
Department of Microbiology, University of Newcastle upon Tyne, UK.
J Gen Microbiol. 1989 Mar;135(3):667-73. doi: 10.1099/00221287-135-3-667.
Adsorption of bacteriophage SP50 to walls and heat-killed cells of Bacillus subtilis 168 appeared to be irreversible at both 37 and 0 degree C. Few, if any, active phage were desorbed when phage-wall complexes, formed at either temperature, were suspended in fresh medium. Bacteria rich in wall teichoic acid (TA) bound phage rapidly at both 0 and 37 degrees C, binding at the higher temperature being approximately twice as fast. Bacteria containing diminished proportions of TA showed less rapid phage adsorption but the reduction in rate was greater at 37 than at 0 degree C and bacteria containing only small proportions of TA bound phage more rapidly at 0 degree C than they did at 37 degrees C. These findings show that at low phage receptor density the temperature affects some component(s) involved in the phage-bacterium interaction such that the collision efficiency is increased at the lower temperature. The possible effect of temperature on the organization of bacterial surface components is discussed.
噬菌体SP50在37℃和0℃时吸附到枯草芽孢杆菌168的细胞壁及热死细胞上似乎都是不可逆的。当在任一温度下形成的噬菌体 - 细胞壁复合物悬浮于新鲜培养基中时，极少有（若有的话）活性噬菌体解吸。富含壁磷壁酸（TA）的细菌在0℃和37℃时都能快速结合噬菌体，在较高温度下的结合速度约为两倍。TA比例降低的细菌显示出较慢的噬菌体吸附，但速率降低在37℃时比在0℃时更大，并且仅含有少量TA的细菌在0℃时比在37℃时更快地结合噬菌体。这些发现表明，在低噬菌体受体密度下，温度会影响噬菌体 - 细菌相互作用中涉及的某些成分，从而在较低温度下碰撞效率会提高。文中讨论了温度对细菌表面成分组织的可能影响。
