Lang W K, Glassey K, Archibald A R
J Bacteriol. 1982 Jul;151(1):367-75. doi: 10.1128/jb.151.1.367-375.1982.
Bacillus subtilis 168 was grown in chemostat culture in fully defined media containing a constant concentration of magnesium and concentrations of phosphate that varied from those giving phosphate-limited growth to those in which phosphate was present in excess and magnesium was limiting. Phosphate-limited bacteria were deficient in wall teichoic acid and contained less than half as much cellular phosphate as did bacteria grown in excess of phosphate. Approximately 70% of the additional phosphate in the latter bacteria was present as wall teichoic acid, indicating that the ability of the bacteria to discontinue teichoic acid synthesis when grown under phosphate limitation permits a substantial increase in their growth yield. Since not all of the additional phosphate is present as wall teichoic acid other cellular phosphates may also be present in reduced amounts in the phosphate-limited bacteria. The content of phosphate groups in walls of magnesium-limited bacteria was similar to the content of uronic acid groups in walls of phosphate-limited bacteria, and walls of bacteria grown in media of intermediate composition contained intermediate proportions of the two anionic polymers. Phage SP50, used as a marker for the presence of teichoic acid, bound densely to nearly all of the bacteria in samples containing down to 22% of the maximum content of teichoic acid. Apparently, therefore, nearly all of these bacteria contain teichoic acid, and the population does not consist of a mixture of individuals having exclusively one kind of anionic polymer. Bacteria containing less than 22% of the maximum content of teichoic bound in a nonuniform manner, and possible explanations for this are discussed.
枯草芽孢杆菌168在恒化器培养中生长，培养基为完全限定培养基，其中镁的浓度恒定，磷酸盐浓度则有所不同，从导致磷酸盐限制生长的浓度到磷酸盐过量而镁限制的浓度。磷酸盐限制条件下的细菌缺乏壁磷壁酸，其细胞内磷酸盐含量不到磷酸盐过量生长细菌的一半。在后者细菌中，约70%的额外磷酸盐以壁磷壁酸形式存在，这表明细菌在磷酸盐限制条件下生长时停止磷壁酸合成的能力使其生长产量大幅增加。由于并非所有额外的磷酸盐都以壁磷壁酸形式存在，磷酸盐限制条件下的细菌中其他细胞磷酸盐的含量可能也会减少。镁限制细菌细胞壁中磷酸基团的含量与磷酸盐限制细菌细胞壁中糖醛酸基团的含量相似，在中间成分培养基中生长的细菌细胞壁含有这两种阴离子聚合物的中间比例。用作磷壁酸存在标记的噬菌体SP50紧密结合到样品中几乎所有细菌上，这些样品中磷壁酸含量低至最大含量的22%。因此，显然几乎所有这些细菌都含有磷壁酸，并且群体并非由仅具有一种阴离子聚合物的个体混合而成。磷壁酸含量低于最大含量22%的细菌结合方式不均匀，并对此进行了可能的解释。
