Archibald A R
J Bacteriol. 1976 Aug;127(2):956-60. doi: 10.1128/jb.127.2.956-960.1976.
Addition of a pulse of excess phosphate to a phosphate-limited culture of Bacillus subtilis W23 resulted in the synthesis and incorporation of wall material that contained teichoic acid. Consequently, the bacteria regained the ability to bind phage SP50 although maximum phage-binding properties did not develop until approximately half a generation time after incorporation of teichoic acid had ceased. The present findings strongly support our earlier suggestion that newly synthesized receptor material is incorporated at the inner surface of the wall and becomes exposed at the outer surface only during subsequent growth.
向枯草芽孢杆菌W23的磷酸盐限制培养物中添加过量磷酸盐脉冲，导致了含有磷壁酸的细胞壁物质的合成与掺入。因此，细菌恢复了结合噬菌体SP50的能力，尽管直到磷壁酸掺入停止约半代时间后才发展出最大的噬菌体结合特性。目前的研究结果有力地支持了我们早期的推测，即新合成的受体物质在细胞壁内表面掺入，并且仅在随后的生长过程中才暴露于外表面。
