[含有重组pUC19质粒的大肠杆菌细胞中β-半乳糖苷酶活性的保存]
[Preservation of the beta-galactosidase activity in E. coli cells containing the recombinant pUC19 plasmid].
作者信息
Paton E B, Zhivolup A N
出版信息
Mol Gen Mikrobiol Virusol. 1987 May(5):19-22.
PMID:3039358
Abstract
Two BglII fragments of pJC703 cosmid were inserted into the plasmid PUC19. E. coli cells containing the recombinant PUC19/A plasmid acquired rifampicin resistance due to inserted rpoB gene but still expressed the Lac+ phenotype.
摘要
将pJC703黏粒的两个BglII片段插入质粒PUC19中。含有重组PUC19/A质粒的大肠杆菌细胞由于插入了rpoB基因而获得了利福平抗性，但仍表现出Lac+表型。
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