Nakano T, Scott P G
Department of Oral Biology, University of Alberta, Edmonton, Canada.
Arch Oral Biol. 1989;34(9):749-57. doi: 10.1016/0003-9969(89)90082-4.
Glycosaminoglycans were prepared from the disc by digestion with papain. The disc contained 5% of its dry weight as glycosaminoglycan. Fractionation by ion-exchange chromatography, followed by precipitation at varying concentrations of ethanol, together with chemical and enzymatic analyses, showed this glycosaminoglycan to consist of approx. 5% hyaluronic acid, 14% dermatan sulphate, 79% chondroitin sulphate and 2% keratan sulphate. Disaccharides obtained from the chondroitin sulphate were 75% 6-sulphated, 21% 4-sulphated and 4% non-sulphated. Chemical analysis showed a low average degree of sulphation of chondroitin sulphate. Immunohistochemical staining of sagittal sections of the disc showed chondroitin sulphate to be distributed throughout, whereas dermatan sulphate (as proteodermatan sulphate) appeared to be concentrated in the periphery.
通过木瓜蛋白酶消化从椎间盘制备糖胺聚糖。该椎间盘含有的糖胺聚糖占其干重的5%。通过离子交换色谱法进行分级分离，随后在不同浓度的乙醇中沉淀，并结合化学和酶分析，结果表明这种糖胺聚糖约由5%的透明质酸、14%的硫酸皮肤素、79%的硫酸软骨素和2%的硫酸角质素组成。从硫酸软骨素获得的二糖中，75%为6-硫酸化、21%为4-硫酸化、4%为非硫酸化。化学分析表明硫酸软骨素的平均硫酸化程度较低。椎间盘矢状切片的免疫组织化学染色显示硫酸软骨素分布于整个椎间盘，而硫酸皮肤素（作为蛋白聚糖硫酸皮肤素）似乎集中在周边。
