Brodskyn C I, Silva A M, Takehara H A, Mota I
Immunology Research and Training Center, Instituto Butantan, São Paulo, Brazil.
Immunol Cell Biol. 1989 Dec;67 ( Pt 6):343-8. doi: 10.1038/icb.1989.50.
To examine the role of different immunoglobulin subclasses in the immune clearance of Trypanosoma cruzi, mice containing bloodstream trypomastigotes were injected intravenously with immune serum, IgG-depleted serum, or with the IgG1 or IgG2 fractions and the rate of removal of the parasites from circulation was determined. Using IgG concentrations similar to those found in the immune serum, the rate of clearance mediated by IgG2 was six-fold higher than that obtained with IgG1. This difference did not appear to be due to differences in antibody specificity, as Western blotting showed that each isotype recognized a similar set of antigens extracted from the parasite. However, the T. cruzi specific antibody content of the IgG2 was approximately five-fold higher than IgG1. When the dose of IgG was adjusted to equalize the antibody content, the clearance ability of the IgG1 and IgG2 was very similar. It is concluded that the two subclasses have a similar clearance ability.
为研究不同免疫球蛋白亚类在克氏锥虫免疫清除中的作用，给含有血流型锥鞭毛体的小鼠静脉注射免疫血清、去除IgG的血清、IgG1或IgG2组分，然后测定寄生虫从循环中清除的速率。使用与免疫血清中相似的IgG浓度，IgG2介导的清除速率比IgG1高6倍。这种差异似乎并非由于抗体特异性不同，因为蛋白质免疫印迹显示每种同种型识别从寄生虫中提取的一组相似的抗原。然而，IgG2中克氏锥虫特异性抗体含量比IgG1高约5倍。当调整IgG剂量以使抗体含量相等时，IgG1和IgG2的清除能力非常相似。得出的结论是，这两个亚类具有相似的清除能力。
