Cachia P G, Pepper D S, Dewar A E, Krajewski A S
Department of Pathology, University of Edinburgh.
J Clin Lab Immunol. 1989 Apr;28(4):195-200.
This paper describes the application of a sensitive ELISA assay detecting as little as 10 ng/ml of a specific idiotope. Using this assay we were able to divide monoclonal anti-idiotypic antibodies generated using a single IgG1 lambda paraprotein as the immunogen into three distinct groups. Seven antibodies detected determinants which were expressed only by the immunogen. The remaining seven antibodies all reacted with normal serum immunoglobulin. Four antibodies reacted with "public" idiotopes which were strongly expressed by serum immunoglobulin, and three antibodies reacted with "restricted public" idiotopes which were only weakly expressed by serum immunoglobulin. This last group may be of significant interest as agents for the immunotherapy of B-cell malignancies. The conservation of expression of these idiotopes in many individual sera suggests that they have a physiological role in immune regulation and would therefore be excellent targets for immunotherapy of B-cell tumours while their quantitatively low expression by circulating immunoglobulin is unlikely to interfere with tumour cell specific binding in vivo.
本文描述了一种灵敏的ELISA检测方法的应用，该方法能够检测低至10 ng/ml的特定独特型表位。利用该检测方法，我们能够将以单一IgG1 λ副蛋白作为免疫原产生的单克隆抗独特型抗体分为三个不同的组。七种抗体检测到仅由免疫原表达的决定簇。其余七种抗体均与正常血清免疫球蛋白发生反应。四种抗体与血清免疫球蛋白强烈表达的“公共”独特型表位发生反应，三种抗体与血清免疫球蛋白仅微弱表达的“受限公共”独特型表位发生反应。最后一组作为B细胞恶性肿瘤免疫治疗的药物可能具有重大意义。这些独特型表位在许多个体血清中的表达保守性表明它们在免疫调节中具有生理作用，因此将是B细胞肿瘤免疫治疗的极佳靶点，而它们在循环免疫球蛋白中的低表达量不太可能在体内干扰肿瘤细胞特异性结合。
