使用单克隆抗独特型抗体识别单克隆抗乙型肝炎病毒表面抗原上的多个独特型表位。
Recognition of several idiotopes on a monoclonal anti-hepatitis B virus surface antigen using monoclonal anti-idiotypic antibodies.
作者信息
Thanavala Y M, Moore M J, Tedder R, Hay F C, Roitt I M
机构信息
Department of Immunology, School of Pathology, Middlesex Hospital Medical School, London, U.K.
出版信息
Immunology. 1988 Apr;63(4):575-7.
A monoclonal murine antibody H3F5 directed to the a determinant of hepatitis B surface antigen (HBsAg) was used to raise several monoclonal anti-idiotypes. Cross-blocking experiments between the anti-idiotypes and the patterns of inhibition produced by a number of other monoclonal anti-HBsAg, generated in the same fusion as H3F5, identified three idiotopes on H3F5 which were shared to varying degrees with the other anti-HBsAg monoclonals. One behaved as a dominant cross-reacting idiotope (CRI) in that it appeared strongly in 5/7 monoclonal idiotypes. The CRI could represent an important target for regulation by anti-idiotope. Monoclonal antibodies have many advantages over polyclonal sera in the detailed analysis of idiotope structures.
一种针对乙型肝炎表面抗原（HBsAg）a决定簇的鼠单克隆抗体H3F5被用于制备多种单克隆抗独特型抗体。抗独特型抗体与许多其他在与H3F5相同的融合中产生的单克隆抗HBsAg所产生的抑制模式之间的交叉阻断实验，确定了H3F5上的三个独特型表位，它们与其他抗HBsAg单克隆抗体有不同程度的共享。其中一个表现为显性交叉反应独特型表位（CRI），因为它在7种单克隆独特型中的5种中强烈出现。该CRI可能是抗独特型调节的重要靶点。在独特型表位结构的详细分析中，单克隆抗体比多克隆血清有许多优势。
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