Hartenstein V, Posakony J W
Department of Biology, University of California San Diego, La Jolla 92093.
Development. 1989 Oct;107(2):389-405. doi: 10.1242/dev.107.2.389.
We have investigated the temporal pattern of appearance, cell lineage, and cytodifferentiation of selected sensory organs (sensilla) of adult Drosophila. This analysis was facilitated by the discovery that the monoclonal antibody 22C10 labels not only the neuron of the developing sensillum organ, but the accessory cells as well. The precursors of the macrochaetes and the recurved (chemosensory) bristles of the wing margin divide around and shortly after puparium formation, while those of the microchaetes and the stout and slender (mechanosensory) bristles of the wing margin divide between 9 h and 18 h after puparium formation (apf). The onset of sensillum differentiation follows the terminal precursor division within a few hours. Four of the cells in an individual microchaete organ are clonally related: A single first-order precursor cell divides to produce two second-order precursors; one of these divides into the neuron and thecogen cell, the other into the trichogen cell and tormogen cell. Along the anterior wing margin, two rounds of division generate the cells of the mechanosensory sensilla; here, no strict clonal relationship seems to exist between the cells of an individual sensillum. At the time of sensillum precursor division, many other, non-sensillum-producing cells within the notum and wing proliferate as well. This mitotic activity follows a spatially non-random pattern.
我们研究了成年果蝇特定感觉器官（感器）出现的时间模式、细胞谱系和细胞分化。单克隆抗体22C10不仅能标记发育中感器器官的神经元，还能标记辅助细胞，这一发现推动了该分析。大刚毛和翅缘弯曲（化学感应）刚毛的前体细胞在蛹形成前后及形成后不久进行分裂，而微刚毛和翅缘粗壮及细长（机械感应）刚毛的前体细胞在蛹形成（apf）后9小时至18小时之间进行分裂。感器分化在终末前体细胞分裂后数小时内开始。单个微刚毛器官中的四个细胞具有克隆相关性：单个一级前体细胞分裂产生两个二级前体细胞；其中一个再分裂为神经元和鞘细胞，另一个分裂为毛原细胞和膜原细胞。沿着翅前缘，两轮分裂产生机械感应感器的细胞；在这里，单个感器的细胞之间似乎不存在严格的克隆关系。在感器前体细胞分裂时，背板和翅内许多其他不产生感器的细胞也会增殖。这种有丝分裂活动遵循空间上的非随机模式。
