Valtorta F, Tarelli F T, Campanati L, Villa A, Greengard P
Bruno Ceccarelli Center for the Study of Peripheral Neuropathies and Neuromuscular Diseases, University of Milano Medical School.
Cell Biol Int Rep. 1989 Dec;13(12):1023-38. doi: 10.1016/0309-1651(89)90017-9.
Synaptophysin, an integral protein of the synaptic vesicle membrane, and synapsin I, a phosphoprotein associated with the cytoplasmic side of synaptic vesicles, represent useful markers that allow to follow the movements of the vesicle membrane during recycling. The use of antibodies against these proteins to label nerve terminals during experimental treatments which stimulate secretion has provided evidence that during the exo-endocytotic cycle synaptic vesicles transiently fuse with the axolemma, from which they are specifically recovered. When recycling is blocked, exocytosis leads to the permanent incorporation of the synaptic vesicle membrane into the axolemma and to diffusion of the vesicle components in the plane of the membrane.
突触小泡蛋白是突触小泡膜的一种整合蛋白，而突触结合蛋白I是一种与突触小泡胞质侧相关的磷蛋白，它们是有用的标志物，可用于追踪回收过程中囊泡膜的运动。在刺激分泌的实验处理过程中，使用针对这些蛋白质的抗体标记神经末梢，这提供了证据表明，在胞吐-内吞循环过程中，突触小泡会短暂地与轴膜融合，并从轴膜中特异性回收。当回收受阻时，胞吐作用会导致突触小泡膜永久性地并入轴膜，并使囊泡成分在膜平面内扩散。
