Nawaz Muhmmad Asif, Bibi Zainab, Aman Afsheen, Zohra Raheela Rahmat, Qader Shah Ali Ul
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
Pak J Pharm Sci. 2014 Sep;27(5 Spec no):1437-42.
Maltase (alpha-glucosidase) hydrolyzes α-(1→4) glucosidic bond of maltose into two glucose molecules. It is widely used in various foods, beverages and also in textile and biofuel industries. During current study, various physicochemical parameters for maltase production from newly isolated strain of Bacillus licheniformis KIBGE-IB4 were optimized using one-factor-at-a-time methodology. It was found that Bacillus licheniformis KIBGE-IB4 produced maximum maltase at 37°C, pH-7.0 after 48 hours using wheat starch (2.5%) as carbon source along with peptone (1.0%), yeast extract (0.2%) and meat extract (0.4%) as nitrogen sources in fermentation medium. It is concluded that the optimization of various medium ingredients and conditions increases maltase production upto 6.74 fold from B. licheniformis KIBGE-IB4 as compared to previously reported media and this strain could be used for the commercial production of maltase.
麦芽糖酶（α-葡萄糖苷酶）将麦芽糖的α-(1→4)糖苷键水解为两个葡萄糖分子。它广泛应用于各种食品、饮料以及纺织和生物燃料行业。在当前研究中，采用一次一个因素的方法对新分离的地衣芽孢杆菌KIBGE-IB4菌株生产麦芽糖酶的各种理化参数进行了优化。结果发现，地衣芽孢杆菌KIBGE-IB4在37°C、pH值为7.0的条件下，使用小麦淀粉（2.5%）作为碳源，同时在发酵培养基中使用蛋白胨（1.0%）、酵母提取物（0.2%）和肉提取物（0.4%）作为氮源，48小时后产生的麦芽糖酶最多。得出的结论是，与先前报道的培养基相比，对各种培养基成分和条件的优化使地衣芽孢杆菌KIBGE-IB4的麦芽糖酶产量提高了6.74倍，该菌株可用于麦芽糖酶的商业生产。
