嗜热芽孢杆菌属菌株产生胞外α-葡萄糖苷酶
Production of extracellular alpha-glucosidase by a thermophilic Bacillus species.
作者信息
Suzuki Y, Kishigami T, Abe S
出版信息
Appl Environ Microbiol. 1976 Jun;31(6):807-12. doi: 10.1128/aem.31.6.807-812.1976.
Abstract
Production of extracellular alpha-glucosidase was studied with strain KP 1006 of a new species of thermophilic Bacillus, which was isolated from soil samples by enrichment at 65 C. alpha-Glucosidase production was maximum at 60 C and at an initial pH of 6.5. The final enzyme yield was increased by starch, maltose, glycerol, peptone, and yeast extract but reduced by acetate and gluconate, alpha-Glucosidase was formed in the cytoplasm and accumulated as a large pool during the logarithmic growth phase. At a midpoint of this period, the enzyme appeared in the culture broth, and its level increased until the end of the stationary phase.
摘要
利用从土壤样品中通过在65℃富集分离得到的嗜热芽孢杆菌新种KP 1006研究了胞外α-葡萄糖苷酶的产生。α-葡萄糖苷酶在60℃和初始pH 6.5时产量最高。淀粉、麦芽糖、甘油、蛋白胨和酵母提取物可提高最终酶产量，但醋酸盐和葡萄糖酸盐会降低产量。α-葡萄糖苷酶在细胞质中形成，并在对数生长期作为一个大的储备池积累。在此阶段的中期，酶出现在培养液中，其水平一直增加到稳定期结束。
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