Department of Dermatology, Jena University Hospital, Jena, Germany; Department of Conservative Dentistry, Jena University Hospital, Jena, Germany; Jena School for Microbial Communication, Friedrich Schiller University of Jena, Jena, Germany.
Department of Dermatology, Jena University Hospital, Jena, Germany.
J Invest Dermatol. 2015 Mar;135(3):750-758. doi: 10.1038/jid.2014.369. Epub 2014 Sep 1.
Dermatophytes initiate dermatophytosis, but susceptibility to infection is dictated by host genetic factors, although the role of some of these-such as human beta-defensin 2 (hBD-2) genomic (DEFB4) copy number (CN) variation and its induction by IL-22-remains unclear. This was investigated in this cross-sectional study in 442 unrelated Caucasian subjects, including 195 healthy controls and 247 dermatophytosis patients who were divided into five subgroups according to clinical presentation. DNA samples were evaluated for DEFB4 CN variation by relative quantification using the comparative CT method, and serum hBD-2 and IL-22 levels were determined by ELISA. DEFB4 CN in patients was significantly lower and, except in the tinea cruris subgroup, serum hBD-2 levels were higher than in controls. The positive correlation between hBD-2 levels and DEFB4 CN observed in controls was not detected in patients, who also had higher serum IL-22 levels that were positively correlated with hBD-2 levels. Moreover, unlike in control subjects, the serum IL-22 level was negatively correlated with DEFB4 CN in patients. Taken together, these findings suggest an association between decreased DEFB4 CN, elevated serum hBD-2 and IL-22 levels, and dermatophytosis, underscoring a gene/cytokine interaction in the occurrence of this infection.
皮肤癣菌引发皮肤癣病，但宿主遗传因素决定了易感性，尽管其中一些因素（如人类β防御素 2 (hBD-2) 基因组 (DEFB4) 拷贝数 (CN) 变异及其受 IL-22 的诱导）的作用尚不清楚。本研究在 442 名无关联的白种人受试者中进行了横断面研究，包括 195 名健康对照者和 247 名皮肤癣病患者，根据临床表现将其分为五个亚组。通过相对定量使用比较 CT 法评估 DEFB4 CN 变异，通过 ELISA 测定血清 hBD-2 和 IL-22 水平。患者的 DEFB4 CN 明显降低，除股癣亚组外，血清 hBD-2 水平也高于对照组。在对照组中观察到的 hBD-2 水平与 DEFB4 CN 之间的正相关在患者中未检测到，而患者的血清 IL-22 水平较高，与 hBD-2 水平呈正相关。此外，与对照组不同，患者的血清 IL-22 水平与 DEFB4 CN 呈负相关。综上所述，这些发现表明 DEFB4 CN 降低、血清 hBD-2 和 IL-22 水平升高与皮肤癣病之间存在关联，突出了这种感染中基因/细胞因子相互作用。
