Department of Dermatology, University Hospitals of Schleswig-Holstein, Kiel, Germany.
Mycoses. 2014 Mar;57(3):147-52. doi: 10.1111/myc.12118. Epub 2013 Aug 19.
In superficial tinea and pityriasis versicolor, the causative fungi are for the most part confined to the stratum corneum which is barely reached by leukocytes. Therefore, a role of non-cellular components in the epidermal antifungal defence was suggested. To investigate the presence of such factors in these infections, the expression of human beta defensins 2 and 3 (hBD-2, hBD-3), RNase 7, psoriasin, toll-like receptors 2, 4 and 9 (TLR2, TLR4 and TLR9) and dectin 2 was analysed by use of immunostainings in skin biopsies. We found that hBD2, hBD3, psoriasin, RNase7, TLR2 and TLR4 were significantly more often expressed in distinct layers of lesional epidermis as compared with uninfected epidermis. In both infections but not in normal skin, hBD2 and hBD3 were commonly expressed within the stratum corneum and in the stratum granulosum. Similarly, psoriasin was seen more often in the upper skin layers of both infections as compared with normal skin. No significant differences between normal and infected skin were found for the expression of TLR9 and dectin 2. Our findings clearly show the expression of specific antimicrobial proteins and defence-related ligands in superficial tinea as well as in pityriasis versicolor, suggesting that these factors contribute to fungal containment.
在浅部真菌病和花斑癣中，大多数致病真菌局限于角质层，白细胞几乎无法到达角质层。因此，人们提出非细胞成分在表皮抗真菌防御中发挥作用。为了研究这些感染中是否存在这些因素，我们通过免疫染色分析了皮肤活检中的人β防御素 2 和 3（hBD-2、hBD-3）、RNase 7、角鲨烯、TLR2、4 和 9（TLR2、TLR4 和 TLR9）和 dectin 2 的表达。我们发现，与未感染的表皮相比，hBD2、hBD3、角鲨烯、RNase7、TLR2 和 TLR4 在病变表皮的不同层中表达更为明显。在这两种感染中，但在正常皮肤中没有，hBD2 和 hBD3 通常在角质层和颗粒层中表达。同样，与正常皮肤相比，角鲨烯在两种感染的上皮肤层中更为常见。TLR9 和 dectin 2 的表达在正常皮肤和感染皮肤之间没有显著差异。我们的研究结果清楚地表明，在浅部真菌病和花斑癣中表达特定的抗菌蛋白和防御相关配体，表明这些因素有助于控制真菌。
