Kuninaka Nobuo, Kawaguchi Minato, Ogawa Masaru, Sato Ayako, Arima Kunimasa, Murayama Shigeo, Saito Yuko
Department of Pathology and Laboratory Medicine, National Center Hospital of Neurology and Psychiatry.
Neuropathology. 2015 Feb;35(1):10-5. doi: 10.1111/neup.12144. Epub 2014 Sep 1.
The Gallyas method is a silver impregnation technique that is essential in the field of neuropathology because of its high sensitivity for the detection of argentophilic inclusion bodies in the central nervous system. In Japan, the Gallyas method has improved and is widely used as the "modified Gallyas method". However, this method is not popularly used in general pathology laboratories because of the need for special reagents, several staining processes, and skilled techniques. The objective of the current study was to provide a simplified Gallyas method. We omitted the lanthanum nitrate step from the staining process and verified the adequacy in comparison with the original method as well as immunohistochemistry, using specimens from patients of Alzheimer's disease, argyrophilic grain disease, multiple system atrophy, Pick's disease, and Lewy body disease. The simplified method provided good staining to all the structures in archival tissues, compared with the modified Gallyas method in a significantly shorter staining time. The lanthanum nitrate step can be omitted from the modified Gallyas method, resulting in reduction in the number of reagents required and shortening of the staining time.
加利亚斯法是一种银浸染技术，因其对检测中枢神经系统嗜银包涵体具有高灵敏度，在神经病理学领域至关重要。在日本，加利亚斯法得到改进并作为“改良加利亚斯法”被广泛使用。然而，由于需要特殊试剂、多个染色步骤以及熟练的技术，该方法在一般病理实验室中并不常用。本研究的目的是提供一种简化的加利亚斯法。我们从染色过程中省略了硝酸镧步骤，并使用阿尔茨海默病、嗜银颗粒病、多系统萎缩、皮克病和路易体病患者的标本，与原方法以及免疫组织化学相比，验证了其充分性。与改良加利亚斯法相比，简化方法在显著更短的染色时间内为存档组织中的所有结构提供了良好的染色效果。改良加利亚斯法可省略硝酸镧步骤，从而减少所需试剂数量并缩短染色时间。
