幼龄犬骨髓和脂肪组织间充质干细胞成骨潜能的比较
Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.
作者信息
Alves Endrigo G L, Serakides Rogéria, Boeloni Jankerle N, Rosado Isabel R, Ocarino Natália M, Oliveira Humberto P, Góes Alfredo M, Rezende Cleuza M F
机构信息
Núcleo de Células Tronco e Terapia Celular Animal, NCT-TCA Departamento de Clínica e Cirurgia, Escola de veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
出版信息
BMC Vet Res. 2014 Sep 2;10:190. doi: 10.1186/s12917-014-0190-y.
BACKGROUND
The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC).
RESULTS
MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points.
CONCLUSIONS
It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.
背景
本研究的目的是比较从幼犬骨髓（BM-MSCs）和脂肪组织（AD-MSCs）中提取的间充质干细胞的成骨潜能。评估了以下参数：噻唑蓝（MTT）转化、碱性磷酸酶（ALP）活性、胶原蛋白和矿化基质合成，以及osterix、骨唾液蛋白（BSP）和骨钙素（OC）的表达。
结果
分化14天和21天后，BM-MSCs的MTT转化率高于AD-MSCs；分化第7天，分化的AD-MSCs的ALP活性更高；第14天和21天，BM-MSCs的胶原蛋白合成更多；与AD-MSCs相比，BM-MSCs每视野矿化面积百分比更高；第14天和21天，BM-MSCs中osterix表达更高，并且在所有研究时间点，BM-MSCs中BSP和OC表达水平更高。
结论
得出的结论是，从幼犬中提取时，BM-MSCs的成骨潜能大于AD-MSCs。
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