Matsuoka Yu, Shimizu Kazuyuki
Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka, 820-8502, Japan.
Methods Mol Biol. 2014;1191:237-60. doi: 10.1007/978-1-4939-1170-7_15.
Conventional metabolic flux analysis (MFA) of Escherichia coli wild type and of pathway gene knockout mutants cultivated under anaerobic condition is explained in detail in this chapter. To place the MFA results into the context of the literature, the regulation of central carbon metabolism in terms of catabolite regulation by the phosphotransferase system (PTS) and the response to oxygen limitations via global regulators is reviewed. The effects of gene deletions such as pflA, pta, ppc, pykF, adhE, and ldhA on the metabolic network are presented. Moreover, for the pflA mutant the effects of various carbon sources were quantified. The chapter thereby contributes to the discussion of metabolic network function and the design of microbial cell factories.
本章详细解释了在厌氧条件下培养的大肠杆菌野生型和途径基因敲除突变体的传统代谢通量分析（MFA）。为了将MFA结果置于文献背景中，本文回顾了磷酸转移酶系统（PTS）的分解代谢调节以及通过全局调节因子对氧限制的响应等中心碳代谢的调节。介绍了诸如pflA、pta、ppc、pykF、adhE和ldhA等基因缺失对代谢网络的影响。此外，对于pflA突变体，还对各种碳源的影响进行了量化。本章有助于对代谢网络功能的讨论以及微生物细胞工厂的设计。
