大肠杆菌的¹³C代谢通量分析

¹³C-metabolic flux analysis for Escherichia coli.

作者信息

Matsuoka Yu, Shimizu Kazuyuki

机构信息

Kyushu Institute of Technology, 680-4 Kawazu, Iizuka-shi, Fukuoka, 820-8502, Japan.

出版信息

Methods Mol Biol. 2014;1191:261-89. doi: 10.1007/978-1-4939-1170-7_16.

DOI:10.1007/978-1-4939-1170-7_16

PMID:25178796

Abstract

(13)C-Metabolic flux analysis ((13)C-MFA) is used here to study the effects of the knockout of such genes as pgi, zwf, gnd, ppc, pck, pyk, and lpdA on the metabolic changes in Escherichia coli cultivated under aerobic condition. The metabolic regulation mechanisms were clarified by integrating such information as fermentation data, gene expression, enzyme activities, and metabolite concentrations as well the result of (13)C-MFA.

摘要

本文采用¹³C代谢通量分析（¹³C-MFA）研究pgi、zwf、gnd、ppc、pck、pyk和lpdA等基因敲除对有氧条件下培养的大肠杆菌代谢变化的影响。通过整合发酵数据、基因表达、酶活性、代谢物浓度等信息以及¹³C-MFA的结果，阐明了代谢调控机制。