Cao Guangyi, Hajisalem Ghazal, Li Wei, Hof Fraser, Gordon Reuven
Electrical and Computer Engineering, University of Victoria, PO box 3065 STN CSC, Victoria, Canada.
Analyst. 2014 Nov 7;139(21):5375-8. doi: 10.1039/c4an01309c.
We quantified an exogenous cancer biomarker, Acetyl amantadine (AcAm), directly from urine solution using surface enhanced Raman spectroscopy (SERS). SERS was used for the detection of AcAm using a commercial Raman substrate after beta-cyclodextrin encapsulation for capture of the analyte. We achieved a detection limit of 1 ng mL(-1) of AcAm in the mock urine in the absence of steroids without extraction or other pre-treatment methods required. With levels of corticosterone typical of urine, the limit of detection was 30 times higher. Since the approach works directly from samples containing the high concentrations of salts and organic co-solutes normal to urine, it has the potential to reduce cost and speed up processing with respect to methods that require pre-purification. Therefore, this is promising for clinical adoption for early cancer detection, particularly for lung cancer.
我们使用表面增强拉曼光谱（SERS）直接从尿液溶液中定量一种外源性癌症生物标志物——乙酰金刚烷胺（AcAm）。在使用β-环糊精包封以捕获分析物后，利用商业拉曼底物通过SERS检测AcAm。在无类固醇且无需提取或其他预处理方法的情况下，我们在模拟尿液中实现了对AcAm 1 ng mL⁻¹的检测限。对于尿液中典型的皮质酮水平，检测限高出30倍。由于该方法直接适用于含有尿液中常见高浓度盐和有机共溶质的样品，相对于需要预纯化的方法，它有可能降低成本并加快处理速度。因此，这对于早期癌症检测，特别是肺癌的临床应用很有前景。
