Desmet Sofie J, Dejager Lien, Clarisse Dorien, Thommis Jonathan, Melchers Diana, Bastiaensen Niek, Ruijtenbeek Rob, Beck Ilse M, Libert Claude, Houtman René, Meijer Onno C, De Bosscher Karolien
Cytokine Receptor Lab, VIB Department of Medical Protein Research, Department of Biochemistry, University of Ghent, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
Methods Mol Biol. 2014;1204:83-94. doi: 10.1007/978-1-4939-1346-6_8.
The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor-coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol, and data analysis.
实时共调节因子-核受体相互作用微阵列分析（MARCoNI）技术可通过流通式微阵列鉴定核受体-共调节因子相互作用。因此，即使在单次实验中，也可以研究不同核受体之间或处于激动剂或拮抗剂模式下的单个受体的共调节因子谱差异。在本章中，将描述如何使用含有过表达糖皮质激素受体的细胞裂解物进行这些肽微阵列实验，以及分析参数的影响、实验方案的变化和数据分析。
