基于聚合酶链反应的耐甲氧西林金黄色葡萄球菌菌株鉴定及其抗生素耐药谱分析
PCR-based identification of methicillin-resistant Staphylococcus aureus strains and their antibiotic resistance profiles.
作者信息
Pournajaf Abazar, Ardebili Abdollah, Goudarzi Leyla, Khodabandeh Mahmoud, Narimani Tahmineh, Abbaszadeh Hassan
机构信息
Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran ; Department of Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
出版信息
Asian Pac J Trop Biomed. 2014 May;4(Suppl 1):S293-7. doi: 10.12980/APJTB.4.2014C423.
OBJECTIVE
To evaluated the PCR for mecA gene compared with the conventional oxacillin disk diffusion method for methicillin-resistant Staphylococcus aureus (S. aureus) identification.
METHODS
A total of 292 S. aureus strains were isolated from various clinical specimens obtained from hospitalized patients. Susceptibility test to several antimicrobial agents was performed by disk diffusion agar according to Clinical and Laboratory Standards Institute guidelines. The PCR amplification of the mecA gene was carried out in all the clinical isolates.
RESULTS
Among antibiotics used in our study, penicillin showed the least anti-staphylococcal activity and vancomycin was the most effective. The rate of methicillin-resistant S. aureus prevalence determined by oxacillin disk diffusion method was 47.6%; whereas, 45.1% of S. aureus isolates were mecA- positive in the PCR assay.
CONCLUSIONS
This study is suggestive that the PCR for detection of mecA gene is a fast, accurate and valuable diagnostic tool, particularly in hospitals in areas where methicillin-resistant S. aureus is endemic.
目的
评估用于检测mecA基因的聚合酶链反应（PCR）与传统苯唑西林纸片扩散法在耐甲氧西林金黄色葡萄球菌（金葡菌）鉴定方面的差异。
方法
从住院患者的各种临床标本中分离出共292株金葡菌。根据临床和实验室标准研究所的指南，采用纸片扩散琼脂法对几种抗菌药物进行药敏试验。对所有临床分离株进行mecA基因的PCR扩增。
结果
在我们研究中使用的抗生素中，青霉素的抗葡萄球菌活性最低，万古霉素最有效。通过苯唑西林纸片扩散法测定的耐甲氧西林金葡菌流行率为47.6%；而在PCR检测中，45.1%的金葡菌分离株mecA呈阳性。
结论
本研究表明，用于检测mecA基因的PCR是一种快速、准确且有价值的诊断工具，尤其在耐甲氧西林金葡菌流行地区的医院。
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