Ishigatsubo Y, Sakamoto H, Hagiwara E, Aoki A, Shirai A, Tani K, Okubo T, Klinman D M
Yokohama City University Medical School, Japan.
Autoimmunity. 1989;5(1-2):71-8. doi: 10.3109/08916938909029144.
An ELISA spot assay was used to quantitate the number of autoantibody-secreting B cells in the peripheral blood of patients with systemic lupus erythematosus. Patients with active disease had 20 fold more anti-DNA, 4 fold more anti-actin and 3 fold more anti-myosin secreting lymphocytes than controls but normal numbers of anti-cardiolipin and anti-transferrin secreting B cells. 60% of SLE patients had increased numbers of B cells reactive with multiple autoantigens. These data suggest that B cell activation in SLE may be influenced by both antigen-specific and antigen-independent factors.
采用酶联免疫斑点试验来定量系统性红斑狼疮患者外周血中分泌自身抗体的B细胞数量。与对照组相比，处于疾病活动期的患者分泌抗DNA、抗肌动蛋白和抗肌球蛋白的淋巴细胞数量分别多出20倍、4倍和3倍，但分泌抗心磷脂和抗转铁蛋白的B细胞数量正常。60%的系统性红斑狼疮患者中，与多种自身抗原发生反应的B细胞数量增多。这些数据表明，系统性红斑狼疮中B细胞的激活可能受抗原特异性和抗原非依赖性因素的影响。
