Klinman D M, Shirai A, Ishigatsubo Y, Conover J, Steinberg A D
Laboratory of Retrovirus Research, Food and Drug Administration, Bethesda, MD 20892.
Arthritis Rheum. 1991 Nov;34(11):1404-10. doi: 10.1002/art.1780341110.
An enzyme-linked immunospot assay was used to quantitate the number of autoantibody-secreting B cells in the peripheral blood of 67 patients with systemic lupus erythematosus. These patients had 1.5-4-fold more lymphocytes secreting IgG and IgM per million peripheral blood lymphocytes than did normal controls. There was a concomitant increase in the number of B cells secreting antibodies reactive with a diverse panel of foreign and self antigens (including actin, myosin, tri-nitrophenylated keyhole limpet hemocyanin, ovalbumin, and retroviral gp160). By comparison, the number of B cells producing anti-DNA antibodies was increased disproportionately. The magnitude of this anti-DNA response correlated significantly with disease activity. Thus, B cell activation in human systemic lupus erythematosus had characteristics of both generalized (polyclonal) B cell activation and (auto)antigen-specific immune stimulation.
采用酶联免疫斑点试验对67例系统性红斑狼疮患者外周血中分泌自身抗体的B细胞数量进行定量分析。这些患者每百万外周血淋巴细胞中分泌IgG和IgM的淋巴细胞数量比正常对照者多1.5至4倍。同时，分泌与多种外源和自身抗原（包括肌动蛋白、肌球蛋白、三硝基苯化血蓝蛋白、卵清蛋白和逆转录病毒gp160）反应性抗体的B细胞数量也有所增加。相比之下，产生抗DNA抗体的B细胞数量增加更为显著。这种抗DNA反应的强度与疾病活动度显著相关。因此，人类系统性红斑狼疮中的B细胞活化具有全身性（多克隆）B细胞活化和（自身）抗原特异性免疫刺激的特征。
