School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia ; Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, Iran.
School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Malaysia.
J Food Sci Technol. 2014 Sep;51(9):1847-56. doi: 10.1007/s13197-012-0742-8. Epub 2012 May 18.
Skin and bone gelatins of pangasius catfish (Pangasius sutchi) were hydrolyzed with alcalase to isolate Angiotensin Converting Enzyme (ACE) inhibitory peptides. Samples with the highest degree of hydrolysis (DH) were separated into different fractions with molecular weight cut-off (MWCO) sizes of 10, 3 and 1 kDa, respectively and assayed for ACE inhibitory activity. Skin and bone gelatins had highest DH of 64.87 and 68.48 % after 2 and 1 h incubation, respectively. Results from this study indicated that by decreasing the molecular weight of fractions, ACE inhibitory activity was increased. Therefore, F3 permeates (MWCO < 1 kDa) of skin (IC50 = 3.2 μg/ml) and bone (IC50 = 1.3 μg/ml) gelatins possessed higher ACE inhibitory activity compared to their untreated gelatins and corresponding hydrolyzed fractions. In this study, the major amino acids were Glycine followed by Proline with an increased amount of hydrophobic amino acid content in F3 permeates of skin (4.01 %) and bone (5.79 %) gelatin. Digestion stability against gastrointestinal proteases did not show any remarkable change on ACE inhibition potency of these permeates. It was concluded that alcalase hydrolysis of P. sutchi by-products could be utilized as a part of functional food or ingredients of a formulated drug in order to control high blood pressure.
利用碱性蛋白酶水解巴沙鱼皮和鱼骨明胶，分离得到血管紧张素转化酶（ACE）抑制肽。对水解度最高的样品进行超滤分离，分别得到截留分子量为 10、3 和 1 kDa 的不同级分，并测定 ACE 抑制活性。经 2 和 1 h 孵育后，皮和骨明胶的水解度最高分别达到 64.87%和 68.48%。结果表明，随着级分分子量的降低，ACE 抑制活性增加。因此，皮（IC50=3.2 μg/ml）和骨（IC50=1.3 μg/ml）明胶的 F3 级分（MWCO<1 kDa）对 ACE 的抑制活性高于未经处理的明胶及其相应的水解级分。在本研究中，主要的氨基酸是甘氨酸，其次是脯氨酸，且 F3 级分中疏水性氨基酸的含量增加，皮（4.01%）和骨（5.79%）明胶均如此。胃肠道蛋白酶消化稳定性对这些级分 ACE 抑制活性没有显著影响。研究结果表明，巴沙鱼副产物经碱性蛋白酶水解后可作为功能性食品的一部分或配方药物的成分，用于控制高血压。
