Ghalamara Soudabeh, Silva Sara, Brazinha Carla, Pintado Manuela
Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua de Diogo Botelho, 1327, 4169-005 Porto, Portugal.
LAQV/Requimte, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
Membranes (Basel). 2020 Mar 13;10(3):44. doi: 10.3390/membranes10030044.
Codfish blood and sardine cooking wastewaters were processed using membrane ultrafiltration that allowed for the preparation of bioactive peptides enriched fractions. The raw materials and corresponding permeates were characterized chemically and in terms of biological properties. The fractionation process was evaluated by analyzing the selective permeation of small peptides (<1 kDa) from larger compounds when using membranes with different molecular weight cut-offs (MWCOs) combined with different materials (MW, PW, and UP010 for codfish blood) and when operated at different transmembrane pressures (with GH for sardine cooking wastewaters). A rejection of the protein/peptides >10 kDa was achieved for both raw materials with the studied membranes. Also, low values of rejection of peptides <1 kDa were accomplished, namely 2% with UP010 from codfish blood and 23% when operated at minimum pressure (1.0 bar) with GH from sardine wastewaters. The peptide fractions from codfish blood with MW and UP010 exhibited the highest ABTS and ORAC values. Peptide fractions from sardine wastewaters with GH demonstrated no improvement in antioxidant activity compared to sardine wastewaters. The antimicrobial results showed that the peptide fractions from codfish blood with UP010 and from sardine with GH at 1.0 bar were capable of inhibiting growth.
使用膜超滤处理鳕鱼血和沙丁鱼烹饪废水，从而制备富含生物活性肽的馏分。对原料和相应的渗透物进行了化学和生物学特性表征。通过分析使用不同截留分子量（MWCO）的膜结合不同材料（鳕鱼血用MW、PW和UP010）以及在不同跨膜压力下运行（沙丁鱼烹饪废水用GH）时小分子肽（<1 kDa）从较大化合物中的选择性渗透，来评估分级过程。对于两种原料，所研究的膜均实现了对>10 kDa蛋白质/肽的截留。此外，对于<1 kDa的肽，截留值较低，即鳕鱼血的UP010截留率为2%，沙丁鱼废水的GH在最低压力（1.0 bar）下运行时截留率为23%。来自鳕鱼血的MW和UP010肽馏分表现出最高的ABTS和ORAC值。与沙丁鱼废水相比，来自沙丁鱼废水的GH肽馏分在抗氧化活性方面没有改善。抗菌结果表明，鳕鱼血的UP010肽馏分和沙丁鱼的GH在1.0 bar时能够抑制生长。
