Takeda M, Obara N, Suzuki Y
Shika Kiso Igakkai Zasshi. 1989 Jun;31(3):317-23. doi: 10.2330/joralbiosci1965.31.317.
The cytoskeletal structures in the apical region of mouse taste bud cells were examined by immunocytochemistry and electron microscopy. Immunostaining for actin showed positive reactions in the apical portions of the taste buds. These regions contained bundles of longitudinally oriented filaments (5-7 nm in diameter) extending from the tip of microvilli to the apical cytoplasm of type I, II, and III cells. After incubation with heavy meromyosin, arrowhead formation was observed along these filaments, thus indicating these filaments to be composed of actin. The plasmalemmal undercoat, which was composed of vertical and horizontal layers, was observed on the zonula occludens. It is supposed that this undercoat gives the structural support for the lateral membrane of the apical region in the taste bud cells.
通过免疫细胞化学和电子显微镜检查了小鼠味蕾细胞顶端区域的细胞骨架结构。肌动蛋白免疫染色显示味蕾顶端部分呈阳性反应。这些区域含有从微绒毛尖端延伸至I型、II型和III型细胞顶端细胞质的纵向排列的细丝束（直径5-7纳米）。用重酶解肌球蛋白孵育后，沿这些细丝观察到箭头状结构，因此表明这些细丝由肌动蛋白组成。在紧密连接上观察到由垂直和水平层组成的质膜内涂层。据推测，这种内涂层为味蕾细胞顶端区域的侧膜提供结构支撑。
