Takeda M, Obara N, Suzuki Y
Department of Anatomy, Higashi Nippon Gakuen University School of Dentistry, Hokkaido, Japan.
Arch Histol Cytol. 1988 Mar;51(1):99-108. doi: 10.1679/aohc.51.99.
The intermediate filaments in mouse taste bud cells were studied by immunocytochemistry using antikeratin antibodies, and by conventional electron microscopy. Taste bud cells (types I, II, and III) possessed less densely aggregated bundles of intermediate filaments than the surrounding epithelial cells. Type III cells, however, contained more densely aggregated bundles than type I or II cells. Basal cells in the taste buds showed aggregations of filaments as dense as those seen in the epithelial cells, although their bundles were more slender than those of the epithelial cells. The antibodies to keratins from the bovine muzzle and human stratum corneum stained all types of the taste bud cells as well as the surrounding epithelial cells. PKK2 antibody reacted with the surrounding epithelial cells, but did not react with the taste bud cells. These results show that keratins are present in both taste bud and surrounding epithelial cells, although the keratin subtype differs between those cells. This finding has led us to the supposition that all cell types comprising the taste buds--including type III (receptor) cells--originate from the epithelial cells surrounding the taste buds. It is also suggested that both keratin subtypes and aggregation patterns of intermediate filament bundles change during differentiation from surrounding epithelial cells to taste bud cells, and from basal cells in the taste buds to types I, II, or III cells.
利用抗角蛋白抗体通过免疫细胞化学方法以及传统电子显微镜技术对小鼠味蕾细胞中的中间丝进行了研究。味蕾细胞（I型、II型和III型）所含的中间丝束聚集程度低于周围的上皮细胞。然而，III型细胞所含的中间丝束聚集程度高于I型或II型细胞。味蕾中的基底细胞显示出与上皮细胞中一样致密的丝聚集，尽管它们的丝束比上皮细胞的更细。来自牛口鼻部和人类角质层的角蛋白抗体可对所有类型的味蕾细胞以及周围的上皮细胞进行染色。PKK2抗体与周围的上皮细胞发生反应，但不与味蕾细胞发生反应。这些结果表明，角蛋白存在于味蕾细胞和周围的上皮细胞中，尽管这些细胞中的角蛋白亚型有所不同。这一发现使我们推测，构成味蕾的所有细胞类型——包括III型（受体）细胞——都起源于味蕾周围的上皮细胞。还表明，在从周围上皮细胞向味蕾细胞以及从味蕾中的基底细胞向I型、II型或III型细胞分化的过程中，角蛋白亚型和中间丝束的聚集模式都会发生变化。
