• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新鉴定出的果蝇G蛋白α0亚基的神经元表达。

Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit.

作者信息

Schmidt C J, Garen-Fazio S, Chow Y K, Neer E J

机构信息

Department of Cardiology, Brigham and Women's Hospital, Boston, Massachusetts 02115.

出版信息

Cell Regul. 1989 Nov;1(1):125-34. doi: 10.1091/mbc.1.1.125.

DOI:10.1091/mbc.1.1.125
PMID:2519611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361431/
Abstract

Guanine nucleotide-binding proteins (G proteins) mediate signals between activated cell-surface receptors and cellular effectors. A bovine G-protein alpha-subunit cDNA has been used to isolate similar sequences from Drosophila genomic and cDNA libraries. One class, which we call DG alpha 0, hybridized to position 47A on the second chromosome of Drosophila. The nucleotide sequence of the protein coding region of one cDNA has been determined, revealing an alpha subunit that is 81% identical with rat alpha 0. The cDNA hybridizes strongly to a 3.8 kb mRNA and weakly with a 5.3 kb message. Antibodies raised against a trp-E-DG alpha 0 fusion protein recognized a 39,000 Da protein in Drosophila extracts. In situ hybridization to adult Drosophila sections combined with immunohistochemical studies revealed expression throughout the optic lobes and central brain and in the thoracic and abdominal ganglia. DG alpha 0 message and protein were also detected in the antennae, oocytes, and ovarian nurse cells. The neuronal expression of this gene is similar to mammalian alpha 0, which is most abundantly expressed in the brain.

摘要

鸟嘌呤核苷酸结合蛋白（G蛋白）介导活化的细胞表面受体与细胞效应器之间的信号传导。一个牛G蛋白α亚基cDNA已被用于从果蝇基因组文库和cDNA文库中分离相似序列。其中一类，我们称之为DGα0，与果蝇第二条染色体上的47A位置杂交。已确定一个cDNA的蛋白质编码区的核苷酸序列，揭示了一个与大鼠α0有81%同一性的α亚基。该cDNA与一个3.8kb的mRNA强烈杂交，与一个5.3kb的信使RNA弱杂交。针对一个trp-E-DGα0融合蛋白产生的抗体在果蝇提取物中识别出一个39000Da的蛋白质。与免疫组织化学研究相结合的对成年果蝇切片的原位杂交显示，该蛋白在整个视叶、中枢脑以及胸神经节和腹神经节中表达。在触角、卵母细胞和卵巢滋养细胞中也检测到了DGα0信使RNA和蛋白质。该基因在神经元中的表达与哺乳动物α0相似，α0在脑中表达最为丰富。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/bcf622cb0918/cellregul00038-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/eb076f2adeae/cellregul00038-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/f1db89e3e33e/cellregul00038-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/9f3138caae14/cellregul00038-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/bcf622cb0918/cellregul00038-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/eb076f2adeae/cellregul00038-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/f1db89e3e33e/cellregul00038-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/9f3138caae14/cellregul00038-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa42/361431/bcf622cb0918/cellregul00038-0140-a.jpg

相似文献

1
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit.新鉴定出的果蝇G蛋白α0亚基的神经元表达。
Cell Regul. 1989 Nov;1(1):125-34. doi: 10.1091/mbc.1.1.125.
2
Molecular characterization of Drosophila gene encoding G0 alpha subunit homolog.
J Biol Chem. 1989 Nov 5;264(31):18536-43.
3
Immunological and molecular characterization of Go alpha-like proteins in the Drosophila central nervous system.果蝇中枢神经系统中Goα样蛋白的免疫学和分子特征
J Biol Chem. 1989 Nov 5;264(31):18552-60.
4
Cloning of a Drosophila melanogaster guanine nucleotide regulatory protein beta-subunit gene and characterization of its expression during development.果蝇黑腹果蝇鸟嘌呤核苷酸调节蛋白β亚基基因的克隆及其在发育过程中的表达特征
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7134-8. doi: 10.1073/pnas.85.19.7134.
5
A Drosophila melanogaster G protein alpha subunit gene is expressed primarily in embryos and pupae.一种黑腹果蝇G蛋白α亚基基因主要在胚胎和蛹中表达。
J Biol Chem. 1988 Aug 25;263(24):12070-6.
6
The Drosophila G protein gamma subunit gene (D-G gamma 1) produces three developmentally regulated transcripts and is predominantly expressed in the central nervous system.果蝇G蛋白γ亚基基因（D-Gγ1）产生三种受发育调控的转录本，且主要在中枢神经系统中表达。
J Biol Chem. 1992 Mar 25;267(9):6086-92.
7
The Drosophila gene coding for the alpha subunit of a stimulatory G protein is preferentially expressed in the nervous system.编码刺激性G蛋白α亚基的果蝇基因在神经系统中优先表达。
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4321-5. doi: 10.1073/pnas.86.11.4321.
8
Sequence analysis of cDNA and genomic DNA for a putative pertussis toxin-insensitive guanine nucleotide-binding regulatory protein alpha subunit.对一种假定的百日咳毒素不敏感的鸟嘌呤核苷酸结合调节蛋白α亚基的cDNA和基因组DNA进行序列分析。
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5384-8. doi: 10.1073/pnas.85.15.5384.
9
The Drosophila Go alpha-like G protein gene produces multiple transcripts and is expressed in the nervous system and in ovaries.
J Biol Chem. 1989 Nov 5;264(31):18544-51.
10
G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.G蛋白多样性：脊椎动物和无脊椎动物中存在一类独特的α亚基。
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9113-7. doi: 10.1073/pnas.87.23.9113.

引用本文的文献

1
Pheromone transduction in moths.信息素转导在蛾类中。
Front Cell Neurosci. 2010 Dec 31;4:133. doi: 10.3389/fncel.2010.00133. eCollection 2010.
2
Go contributes to olfactory reception in Drosophila melanogaster.Go蛋白在黑腹果蝇的嗅觉接收中发挥作用。
BMC Physiol. 2009 Nov 28;9:22. doi: 10.1186/1472-6793-9-22.
3
Human T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling.人类T细胞白血病病毒1型Tax癌蛋白调节G蛋白信号传导。

本文引用的文献

1
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.荧光显微镜检查：没食子酸正丙酯减少罗丹明和荧光素蛋白缀合物的光漂白
Science. 1982 Sep 24;217(4566):1252-5. doi: 10.1126/science.7112126.
2
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
Anal Biochem. 1983 Jul 1;132(1):6-13. doi: 10.1016/0003-2697(83)90418-9.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Blood. 2007 Feb 1;109(3):1051-60. doi: 10.1182/blood-2006-06-026781. Epub 2006 Sep 21.
4
The heterotrimeric protein Go is required for the formation of heart epithelium in Drosophila.异源三聚体蛋白Go是果蝇心脏上皮形成所必需的。
J Cell Biol. 1999 May 31;145(5):1063-76. doi: 10.1083/jcb.145.5.1063.
5
G alpha(o) is necessary for muscarinic regulation of Ca2+ channels in mouse heart.Gα(o) 对于毒蕈碱对小鼠心脏中钙离子通道的调节是必需的。
Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1727-32. doi: 10.1073/pnas.94.5.1727.
6
Characterization of a GTP-binding protein implicated in both memory storage and interorganelle vesicle transport.一种与记忆存储和细胞器间囊泡运输均有关的GTP结合蛋白的特性分析。
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9287-91. doi: 10.1073/pnas.91.20.9287.
7
G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.G蛋白多样性：脊椎动物和无脊椎动物中存在一类独特的α亚基。
Proc Natl Acad Sci U S A. 1990 Dec;87(23):9113-7. doi: 10.1073/pnas.87.23.9113.
8
Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans.线虫秀丽隐杆线虫中的同源和独特G蛋白α亚基。
Cell Regul. 1991 Feb;2(2):135-54. doi: 10.1091/mbc.2.2.135.
9
G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits.Gα12和Gα13亚基定义了G蛋白α亚基的第四类。
Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5582-6. doi: 10.1073/pnas.88.13.5582.
10
Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.可变剪接产生编码GTP结合蛋白Goα亚基两种形式的转录本。
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6477-81. doi: 10.1073/pnas.87.17.6477.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy.高碘酸盐-赖氨酸-多聚甲醛固定剂。一种用于免疫电子显微镜的新型固定剂。
J Histochem Cytochem. 1974 Dec;22(12):1077-83. doi: 10.1177/22.12.1077.
5
Physical and immunological characterization of a guanine nucleotide-binding protein purified from bovine cerebral cortex.从牛大脑皮层纯化的一种鸟嘌呤核苷酸结合蛋白的物理和免疫学特性
J Biol Chem. 1985 Sep 5;260(19):10864-71.
6
Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray crystallography.通过X射线晶体学研究鸟苷二磷酸与大肠杆菌延伸因子Tu结合的结构细节。
EMBO J. 1985 Sep;4(9):2385-8. doi: 10.1002/j.1460-2075.1985.tb03943.x.
7
Structure of the GDP domain of EF-Tu and location of the amino acids homologous to ras oncogene proteins.EF-Tu的GDP结构域的结构以及与ras癌基因蛋白同源的氨基酸的位置。
Science. 1985 Oct 4;230(4721):32-6. doi: 10.1126/science.3898365.
8
An alpha 40 subunit of a GTP-binding protein immunologically related to Go mediates a dopamine-induced decrease of Ca2+ current in snail neurons.一种与Go免疫相关的GTP结合蛋白的α40亚基介导了蜗牛神经元中多巴胺诱导的Ca2+电流降低。
Neuron. 1988 Mar;1(1):27-32. doi: 10.1016/0896-6273(88)90206-1.
9
Cloning of a Drosophila melanogaster guanine nucleotide regulatory protein beta-subunit gene and characterization of its expression during development.果蝇黑腹果蝇鸟嘌呤核苷酸调节蛋白β亚基基因的克隆及其在发育过程中的表达特征
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7134-8. doi: 10.1073/pnas.85.19.7134.
10
A Drosophila melanogaster G protein alpha subunit gene is expressed primarily in embryos and pupae.一种黑腹果蝇G蛋白α亚基基因主要在胚胎和蛹中表达。
J Biol Chem. 1988 Aug 25;263(24):12070-6.