Feldmann A M, Ponnudurai T
Research Institute ITAL, Wageningen, The Netherlands.
Med Vet Entomol. 1989 Jan;3(1):41-52. doi: 10.1111/j.1365-2915.1989.tb00473.x.
Variation in susceptibility of the vector Anopheles stephensi Liston to the human malaria parasite Plasmodium falciparum (Welch) was demonstrated using twelve strains of mosquitoes and one strain of parasites cultured in vitro. The Beech strain of An. stephensi exhibited greatest natural refractoriness, but with high intrapopulation variability. By selection for the required characteristic, two refractory lines of the Punjab strain and one highly susceptible line of the Sind strain were obtained. The median number of oocysts in the two refractory lines was less than 4% of that in the unselected line, whilst the highly susceptible line yielded about twice as many oocysts as the unselected line. Selection progressed more by keeping the descendants of individual females separate and selecting between them (individual selection) rather than pooling the progeny of all selected mosquitoes (mass selection). Using the former procedure many lines were lost due to inbreeding depression, but the outcome was more successful.
利用12株按蚊和1株体外培养的疟原虫，证明了媒介斯氏按蚊（Liston）对人类疟原虫恶性疟原虫（Welch）的易感性存在差异。斯氏按蚊的Beech品系表现出最大的天然抗性，但群体内变异性高。通过选择所需特性，获得了旁遮普品系的两个抗性品系和信德品系的一个高敏感品系。两个抗性品系中卵囊的中位数不到未选择品系的4%，而高敏感品系产生的卵囊数量约为未选择品系的两倍。通过将单个雌性的后代分开并在它们之间进行选择（个体选择），而不是将所有选定蚊子的后代集中在一起（群体选择），选择进展更大。使用前一种方法，许多品系因近亲繁殖衰退而丢失，但结果更成功。
