Vakili Bahareh, Khorvash Farzin, Fazeli Hossein, Khaleghi Moj
Department of Microbiology, Science and Research Branch, Islami Azad University, Kerman, Iran.
Assistant Professor, Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
J Res Med Sci. 2014 Jun;19(6):567-70.
The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran.
Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation.
77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates.
High proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.
本研究旨在筛查来自伊朗伊斯法罕阿尔扎赫拉医院重症监护病房（ICU）的鲍曼不动杆菌临床分离株中的parC基因突变情况。
对2011年3月至2012年6月期间的70株鲍曼不动杆菌进行了研究。采用E-test法进行药敏试验。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和测序检测parC基因突变。
77.1%的标本具有高度耐药性。在93%的分离株中观察到parC基因第80位发生突变。
高比例的鲍曼不动杆菌分离株存在parC基因突变，这可能在这些分离株发病率增加中起重要作用。
