暴露于二氧化氯气体4小时会使鼠管状线虫卵失去活力。
Exposure to chlorine dioxide gas for 4 hours renders Syphacia ova nonviable.
作者信息
Czarra Jane A, Adams Joleen K, Carter Christopher L, Hill William A, Coan Patricia N
机构信息
Office of Laboratory Animal Care, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee, USA.
Animal Care Unit, Office of Research, The University of Kansas, Lawrence, Kansas, USA; Department of Pharmacology and Toxicology, The University of Kansas, Lawrence, Kansas, USA.
出版信息
J Am Assoc Lab Anim Sci. 2014 Jul;53(4):364-7.
The purpose of our study was to evaluate the efficacy of chlorine dioxide gas for environmental decontamination of Syphacia spp. ova. We collected Syphacia ova by perianal cellophane tape impression of pinworm-infected mice. Tapes with attached ova were exposed to chlorine dioxide gas for 1, 2, 3, or 4 h. After gas exposure, ova were incubated in hatching medium for 6 h to promote hatching. For controls, tapes with attached ova were maintained at room temperature for 1, 2, 3, and 4 h without exposure to chlorine dioxide gas and similarly incubated in hatch medium for 6 h. Ova viability after incubation was assessed by microscopic examination. Exposure to chlorine dioxide gas for 4 h rendered 100% of Syphacia spp. ova nonviable. Conversely, only 17% of ova on the 4-h control slide were nonviable. Other times of exposure to chlorine dioxide gas resulted in variable effectiveness. These data suggest that exposure to chlorine dioxide gas for at least 4 h is effective for surface decontamination of Syphacia spp. ova.
我们研究的目的是评估二氧化氯气体对环境中鼠管状线虫属虫卵的去污效果。我们通过对感染蛲虫的小鼠进行肛周透明胶带粘贴来收集鼠管状线虫虫卵。带有虫卵的胶带分别暴露于二氧化氯气体中1、2、3或4小时。气体暴露后，将虫卵在孵化培养基中孵育6小时以促进孵化。作为对照，带有虫卵的胶带在室温下放置1、2、3和4小时，不暴露于二氧化氯气体，然后同样在孵化培养基中孵育6小时。孵育后通过显微镜检查评估虫卵的活力。暴露于二氧化氯气体4小时后，100%的鼠管状线虫属虫卵失去活力。相反，4小时对照载玻片上只有17%的虫卵失去活力。其他暴露于二氧化氯气体的时间产生了不同的效果。这些数据表明，暴露于二氧化氯气体至少4小时对鼠管状线虫属虫卵的表面去污是有效的。
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