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鼠管状线虫虫卵销毁方法的评估。

Assessment of methods of destruction of Syphacia muris eggs.

作者信息

Dix J, Astill J, Whelan G

机构信息

Department of Laboratory Animal Science, GlaxoSmithKline Pharmaceuticals, The Frythe, Welwyn, Hertfordshire AL6 9AR, UK.

出版信息

Lab Anim. 2004 Jan;38(1):11-6. doi: 10.1258/00236770460734344.

Abstract

An investigation was performed to identify methods suitable for decontamination of equipment being transferred from a facility contaminated with Syphacia muris to a new facility. Perianal samples were taken on clear sticky tape from rats known to be infected with S. muris. Tapes and attached worm eggs were treated with ethylene oxide, formaldehyde fumigation, potassium peroxysulphate, chlorine dioxide and didecyl di-methyl ammonium chloride, chlorine dioxide, alcohol/chlorhexidine, 100 degrees C dry heat for 30 min, ultraviolet light (UV), or left for 4 weeks at room temperature. The subsequent viability of the eggs was compared to untreated control samples. Heat and ethylene oxide produced a 100% kill rate of S. muris eggs. Formaldehyde gas and chlorine dioxide treatments showed marked effectiveness, with the number of eggs killed at 94% and 96%, respectively, while the other agents showed less efficacy (36-78% eggs killed). The percentage of eggs not hatching in the controls was 21%. Since so few agents were effective against S. muris eggs, care should be taken when choosing a method for decontaminating a facility.

摘要

开展了一项调查,以确定适用于将受鼠管状线虫污染的设施中的设备转移至新设施时进行去污的方法。从已知感染鼠管状线虫的大鼠的肛周采集透明胶带样本。将胶带及附着的虫卵分别用环氧乙烷、甲醛熏蒸、过硫酸钾、二氧化氯、双癸基二甲基氯化铵、二氧化氯、酒精/洗必泰、100℃干热30分钟、紫外线(UV)处理,或在室温下放置4周。将处理后虫卵的后续活力与未处理的对照样本进行比较。加热和环氧乙烷对鼠管状线虫虫卵的杀灭率达100%。甲醛气体和二氧化氯处理显示出显著效果,杀死的虫卵数量分别为94%和96%,而其他试剂的效果较差(杀死的虫卵为36 - 78%)。对照中未孵化的虫卵百分比为21%。由于很少有试剂对鼠管状线虫虫卵有效,因此在选择设施去污方法时应谨慎。

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