Slate Andrea R, Bandyopadhyay Sheila, Francis Kevin P, Papich Mark G, Karolewski Brian, Hod Eldad A, Prestia Kevin A
Division of Comparative Medicine, University of South Florida, Tampa, Florida, USA.
Laboratory of Transfusion Biology, Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, New York, USA.
J Am Assoc Lab Anim Sci. 2014 Jul;53(4):381-6.
We examined the efficacy of enrofloxacin administered by 2 different routes in a mouse model of sepsis. Male CD1 mice were infected with a bioluminescent strain of enteropathogenic Escherichia coli and treated with enrofloxacin either by injection or in drinking water. Peak serum levels were evaluated by using HPLC. Mice were monitored for signs of clinical disease, and infections were monitored by using bioluminescence imaging. Serum levels of enrofloxacin and the active metabolite ciprofloxacin were greater in the group treated by injection than in controls or the groups treated by administration in drinking water. Survival of the group treated with enrofloxacin injection was greater than that of controls and groups treated with enrofloxacin in the drinking water. Bioluminescence in the group treated with enrofloxacin injection was less than that in the groups treated with oral administration at 12 h and in the groups treated orally and the control group at 16 h. According to these findings, we recommend the use of injectable enrofloxacin at 5 mg/kg SC for mice with systemic infections.
我们在脓毒症小鼠模型中研究了通过两种不同途径给予恩诺沙星的疗效。雄性CD1小鼠感染了一株表达生物荧光的肠道致病性大肠杆菌，然后通过注射或饮水方式给予恩诺沙星进行治疗。使用高效液相色谱法评估血清峰值水平。监测小鼠的临床疾病体征，并使用生物发光成像监测感染情况。注射给药组的恩诺沙星和活性代谢物环丙沙星的血清水平高于对照组或饮水给药组。恩诺沙星注射治疗组的存活率高于对照组和饮水给予恩诺沙星的组。在12小时时，恩诺沙星注射治疗组的生物发光低于口服给药组，在16小时时低于口服给药组和对照组。根据这些发现，我们建议对于有全身感染的小鼠，以5mg/kg皮下注射的方式使用注射用恩诺沙星。
