Felt Stephen, Papich Mark G, Howard Antwain, Long Tyler, McKeon Gabriel, Torreilles Stéphanie, Green Sherril
Comparative Medicine, Stanford University School of Medicine, Stanford, CA, USA.
J Am Assoc Lab Anim Sci. 2013 Mar;52(2):186-8.
As part of an enrofloxacin pharmacokinetic study, concentrations of enrofloxacin and ciprofloxacin (metabolite) were measured in various tissues (brain, heart, kidney, liver, lung, and spleen) collected from treated (subcutaneous delivery, n = 3; intramuscular delivery, n = 3; untreated controls, n = 2) adult female Xenopus laevis by using HPLC. Enrofloxacin was rapidly absorbed after administration by either route and readily diffused into all sampled tissues. Enrofloxacin and ciprofloxacin were present in the tissue samples collected at 8 h. The highest average tissue concentrations for enrofloxacin were found in kidney, with the lowest concentrations in liver. Ciprofloxacin tissue concentrations paralleled but were always lower than those of enrofloxacin for all time points and tissues except brain and kidney. These results, together with previously published pharmacokinetic data and known minimal inhibitory concentrations of common pathogenic bacteria, provide a strong evidence-based rationale for choosing enrofloxacin to treat infectious diseases in X. laevis.
作为恩诺沙星药代动力学研究的一部分，通过高效液相色谱法测定了从接受治疗（皮下给药，n = 3；肌肉注射，n = 3；未治疗对照组，n = 2）的成年雌性非洲爪蟾采集的各种组织（脑、心脏、肾脏、肝脏、肺和脾脏）中恩诺沙星和环丙沙星（代谢物）的浓度。恩诺沙星经任一途径给药后均迅速吸收，并易于扩散到所有采样组织中。在8小时采集的组织样本中存在恩诺沙星和环丙沙星。恩诺沙星的平均组织浓度在肾脏中最高，在肝脏中最低。除脑和肾脏外，环丙沙星在所有时间点和组织中的组织浓度均与恩诺沙星平行，但始终低于恩诺沙星。这些结果，连同先前发表的药代动力学数据和常见病原菌已知的最低抑菌浓度，为选择恩诺沙星治疗非洲爪蟾的传染病提供了强有力的循证依据。
