Abi-Ghanem Josephine, Gabelica Valérie
CNRS, UMS 3033, IECB, F-33600 Pessac, France.
Phys Chem Chem Phys. 2014 Oct 21;16(39):21204-18. doi: 10.1039/c4cp02362e. Epub 2014 Sep 9.
Nucleic acids are diverse polymeric macromolecules that are essential for all life forms. These biomolecules possess a functional three-dimensional structure under aqueous physiological conditions. Mass spectrometry-based approaches have on the other hand opened the possibility to gain structural information on nucleic acids from gas-phase measurements. To correlate gas-phase structural probing results with solution structures, it is therefore important to grasp the extent to which nucleic acid structures are preserved, or altered, when transferred from the solution to a fully anhydrous environment. We will review here experimental and theoretical approaches available to characterize the structure of nucleic acids in the gas phase (with a focus on oligonucleotides and higher-order structures), and will summarize the structural features of nucleic acids that can be preserved in the gas phase on the experiment time scale.
核酸是多种多样的聚合大分子，对所有生命形式都至关重要。这些生物分子在水性生理条件下具有功能性三维结构。另一方面，基于质谱的方法为从气相测量中获取核酸结构信息提供了可能性。为了将气相结构探测结果与溶液结构相关联，因此重要的是要了解核酸结构从溶液转移到完全无水环境时被保留或改变的程度。我们将在此回顾可用于表征气相中核酸结构的实验和理论方法（重点是寡核苷酸和高阶结构），并总结在实验时间尺度上可在气相中保留的核酸结构特征。
